CHARACTERIZATION OF A YEAST ARTIFICIAL CHROMOSOME CONTIG SPANNING THE HUNTINGTONS-DISEASE GENE CANDIDATE REGION

被引：76

作者：

BATES, GP

VALDES, J

HUMMERICH, H

BAXENDALE, S

LEPASLIER, DL

MONACO, AP

TAGLE, D

MACDONALD, ME

ALTHERR, M

ROSS, M

BROWNSTEIN, BH

BENTLEY, D

WASMUTH, JJ

GUSELLA, JF

COHEN, D

COLLINS, F

LEHRACH, H

机构：

[1] CTR ETUD POLYMORPHISME HUMAIN,F-75010 PARIS,FRANCE

[2] UNIV MICHIGAN,HOWARD HUGHES MED INST,ANN ARBOR,MI 48109

[3] UNIV MICHIGAN,DEPT INTERNAL MED & HUMAN GENET,ANN ARBOR,MI 48109

[4] JOHN RADCLIFFE HOSP,INST MOLEC MED,IMPERIAL CANC RES FUND,HUMAN GENET LAB,OXFORD OX3 9DU,ENGLAND

[5] MASSACHUSETTS GEN HOSP,NEUROGENET LAB,BOSTON,MA 02114

[6] HARVARD UNIV,SCH MED,DEPT GENET,BOSTON,MA 02115

[7] UNIV CALIF IRVINE,COLL MED,DEPT BIOL CHEM,IRVINE,CA 92717

[8] WASHINGTON UNIV,SCH MED,DEPT GENET,ST LOUIS,MO 63110

[9] GUYS HOSP,DIV MED & MOLEC GENET,LONDON SE1 9RT,ENGLAND

来源：

NATURE GENETICS | 1992年 / 1卷 / 03期

关键词：

D O I：

10.1038/ng0692-180

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The Huntington's disease (HD) gene has been localized by recombination events to a region covering 2.2 megabases (Mb) DNA within chromosome 4p16.3. We have screened three yeast artificial chromosome (YAC) libraries in order to isolate and characterize 44 YAC clones mapping to this region. Approximately 50% of the YACs were chimaeric. Unstable YACs were identified across the whole region, but were particularly prevalent around the D4S183 and D4S43 loci. The YACs have been assembled into a contig extending from D4S126 to D4S98 covering roughly 2 Mb DNA except for a gap of about 250 kilobases (kb). The establishment of a YAC contig which spans the region most likely to contain the HD mutation is an essential step in the isolation of the HD gene.

引用

页码：180 / 187

页数：8

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