DISSOCIATION OF RECA FILAMENTS FROM DUPLEX DNA BY THE RUVA AND RUVB DNA-REPAIR PROTEINS

被引：36

作者：

ADAMS, DE ^{[1
]}

TSANEVA, IR ^{[1
]}

WEST, SC ^{[1
]}

机构：

[1] IMPERIAL CANC RES FUND,CLARE HALL LABS,S MIMMS EN6 3LD,HERTS,ENGLAND

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 21期

关键词：

RECOMBINATION; BRANCH MIGRATION; HELICASE; TOPOISOMERASE; DNA SUPERCOILING;

D O I：

10.1073/pnas.91.21.9901

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The RuvA and RuvB proteins of Escherichia coli act late in recombination and DNA repair to catalyze the branch migration of Holliday junctions made by RecA. In this paper, we show that addition of RuvAB to supercoiled DNA that is bound by RecA leads to the rapid dissociation of the RecA nucleoprotein filament, as determined by a topological assay that measures DNA underwinding and a restriction endonuclease protection assay. Disruption of the RecA filament requires RuvA, RuvB, and hydrolysis of ATP. These findings suggest several important roles for the RuvAB helicase during genetic recombination and DNA repair: (i) displacement of RecA filaments from double-stranded DNA, (ii) interruption of RecA-mediated strand exchange, (iii) RuvAB-catalyzed branch migration, and (iv) recycling of RecA protein.

引用

页码：9901 / 9905

页数：5

共 28 条

[21] THE ESCHERICHIA-COLI RUVB BRANCH MIGRATION PROTEIN FORMS DOUBLE HEXAMERIC RINGS AROUND DNA [J].

STASIAK, A ;

TSANEVA, IR ;

WEST, SC ;

BENSON, CJB ;

YU, X ;

EGELMAN, EH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (16) :7618-7622

[22] PURIFICATION AND PROPERTIES OF THE RUVA AND RUVB PROTEINS OF ESCHERICHIA-COLI [J].

TSANEVA, IR ;

ILLING, G ;

LLOYD, RG ;

WEST, SC .

MOLECULAR & GENERAL GENETICS, 1992, 235 (01) :1-10

[23] ATP-DEPENDENT BRANCH MIGRATION OF HOLLIDAY JUNCTIONS PROMOTED BY THE RUVA AND RUVB PROTEINS OF ESCHERICHIA-COLI [J].

TSANEVA, IR ;

MULLER, B ;

WEST, SC .

CELL, 1992, 69 (07) :1171-1180

[24] RUVA AND RUVB PROTEINS OF ESCHERICHIA-COLI EXHIBIT DNA HELICASE ACTIVITY INVITRO [J].

TSANEVA, IR ;

MULLER, B ;

WEST, SC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (04) :1315-1319

[25] THE PROCESSING OF RECOMBINATION INTERMEDIATES - MECHANISTIC INSIGHTS FROM STUDIES OF BACTERIAL PROTEINS [J].

WEST, SC .

CELL, 1994, 76 (01) :9-15

[26] ENZYMES AND MOLECULAR MECHANISMS OF GENETIC-RECOMBINATION [J].

WEST, SC .

ANNUAL REVIEW OF BIOCHEMISTRY, 1992, 61 :603-640

[27] UNWINDING ASSOCIATED WITH SYNAPSIS OF DNA-MOLECULES BY RECA PROTEIN [J].

WU, AM ;

BIANCHI, M ;

DASGUPTA, C ;

RADDING, CM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (05) :1256-1260

[28] BOUND LAC REPRESSOR PROTEIN DIFFERENTIALLY INHIBITS THE UNWINDING REACTIONS CATALYZED BY DNA HELICASES [J].

YANCEYWRONA, JE ;

MATSON, SW .

NUCLEIC ACIDS RESEARCH, 1992, 20 (24) :6713-6721

← 1 2 3 →