A SOLID-PHASE ENZYME-LINKED ASSAY FOR INFLUENZA-VIRUS RECEPTOR-BINDING ACTIVITY

被引：111

作者：

GAMBARYAN, AS ^{[1
]}

MATROSOVICH, MN ^{[1
]}

机构：

[1] RUSSIAN ACAD MED SCI,INST POLIOMYELITIS & VIRAL ENCEPHALITIDES,MOSCOW 142782,USSR

来源：

JOURNAL OF VIROLOGICAL METHODS | 1992年 / 39卷 / 1-2期

关键词：

INFLUENZA VIRUS; VIRUS RECEPTOR-BINDING ACTIVITY; VIRUS INHIBITOR; SIALIC ACID; SIALYLGLYCOPROTEIN; SOLID-PHASE ENZYME-LINKED ASSAY;

D O I：

10.1016/0166-0934(92)90130-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Receptor-binding properties of influenza viruses are usually characterized by the ability of viruses to interact with more or less defined sialic acid-containing carbohydrates, glycoproteins, or glycolipids assayed by haemagglutination (HA) or HA inhibition (HAI) tests. To overcome some drawbacks of these tests a solid-phase enzyme linked assay analogous to sandwich ELISA was developed. The virus is adsorbed specifically to the well of plastic microtitre plates coated with fetuin, and the binding of horseradish peroxidase (HRP)labelled sialylglycoproteins (SGPs) by the solid phase-attached virions is measured. The binding of unlabelled compounds is measured by competition with the attachment of a standard fetuin-HRP conjugate. The assay is easy to perform, quantitative (allows the determination of affinity constants), and sensitive (even the weak binding of free N-acetylneuraminic acid with K(d) about 10(-1)-10(-2) M-1 can be studied). Due to a higher stability of components of the present test system, as compared to red blood cells, the influence of pH, ionic strength, and other factors on virus-receptor interaction can also be investigated.

引用

页码：111 / 123

页数：13

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