A RAPID AND SIMPLE ONE-STEP METHOD FOR ISOLATION OF POLY(A)+ RNA FROM CELLS IN MONOLAYER

被引：61

作者：

HARTMANN, KKP

PAPA, V

BROWN, EJ

DOERRIES, U

ROSENTHAL, SM

GOLDFINE, ID

机构：

[1] MT ZION HOSP & MED CTR,DEPT MED,SAN FRANCISCO,CA 94120

[2] UNIV CALIF SAN FRANCISCO,DEPT MED,SAN FRANCISCO,CA 94143

[3] UNIV CALIF SAN FRANCISCO,DEPT PHYSIOL,SAN FRANCISCO,CA 94143

[4] UNIV CALIF SAN FRANCISCO,DEPT PEDIAT,SAN FRANCISCO,CA 94143

[5] UNIV HEIDELBERG,DEPT NEUROBIOL,W-6900 HEIDELBERG,GERMANY

来源：

ENDOCRINOLOGY | 1990年 / 127卷 / 04期

关键词：

D O I：

10.1210/endo-127-4-2038

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A simple method was developed to isolate low abundance hormone receptor poly(A)+ RNA from cells in tissue culture. Adherent cells in tissue culture plates were directly released with proteinase K and solubilized in SDS. Oligo(dT) cellulose was directly added to the lysate to obtain poly(A)+ RNA. Yields and purity of the poly(A)+ RNA were comparable to other more lengthy methods. IGF-I receptor and insulin receptor mRNA could be detected on Northern blot without any degradation. © 1990 by The Endocrine Society.

引用

页码：2038 / 2040

页数：3

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