EFFECT OF PH ON LOW-DENSITY-LIPOPROTEIN OXIDATION BY O2-/HO2. FREE-RADICALS PRODUCED BY GAMMA-RADIOLYSIS

被引：36

作者：

BONNEFONTROUSSELOT, D ^{[1
]}

GARDESALBERT, M ^{[1
]}

LEPAGE, S ^{[1
]}

DELATTRE, J ^{[1
]}

FERRADINI, C ^{[1
]}

机构：

[1] UNIV PARIS 05, CHIM PHYS LAB, CNRS, URA 400, F-75270 PARIS 06, FRANCE

来源：

RADIATION RESEARCH | 1992年 / 132卷 / 02期

关键词：

D O I：

10.2307/3578530

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

This study aimed to analyze quantitatively the initiation and the consequences of low-density lipoprotein (LDL) peroxidation by O2/- ·/HO2· free radicals produced by gamma radiolysis. The action of increasing radiation doses on aqueous LDL solutions has been monitored simultaneously by several parameters: a decrease in endogenous vitamin E, the formation of thiobarbituric acid-reactive substances (TBARS) and conjugated dienes, the appearance of a differential fluorescence (excitation wavelength = 360 nm), and an increase of the relative electrophoretic mobility. Initial radiation yields (decrease in vitamin E, formation of TBARS) have been determined at pH 7 and pH 5.7 as a function of LDL concentration (from 0.75 to 9 g liter-1). From the comparison of these yields with those of O2/- radicals produced by water radiolysis, we have deduced reaction mechanisms for LDL peroxidation initiated by O2-·/HO2· free radicals.

引用

页码：228 / 236

页数：9