EFFECT OF PH ON LOW-DENSITY-LIPOPROTEIN OXIDATION BY O2-/HO2. FREE-RADICALS PRODUCED BY GAMMA-RADIOLYSIS

被引：36

作者：

BONNEFONTROUSSELOT, D ^{[1
]}

GARDESALBERT, M ^{[1
]}

LEPAGE, S ^{[1
]}

DELATTRE, J ^{[1
]}

FERRADINI, C ^{[1
]}

机构：

[1] UNIV PARIS 05, CHIM PHYS LAB, CNRS, URA 400, F-75270 PARIS 06, FRANCE

来源：

RADIATION RESEARCH | 1992年 / 132卷 / 02期

关键词：

D O I：

10.2307/3578530

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

This study aimed to analyze quantitatively the initiation and the consequences of low-density lipoprotein (LDL) peroxidation by O2/- ·/HO2· free radicals produced by gamma radiolysis. The action of increasing radiation doses on aqueous LDL solutions has been monitored simultaneously by several parameters: a decrease in endogenous vitamin E, the formation of thiobarbituric acid-reactive substances (TBARS) and conjugated dienes, the appearance of a differential fluorescence (excitation wavelength = 360 nm), and an increase of the relative electrophoretic mobility. Initial radiation yields (decrease in vitamin E, formation of TBARS) have been determined at pH 7 and pH 5.7 as a function of LDL concentration (from 0.75 to 9 g liter-1). From the comparison of these yields with those of O2/- radicals produced by water radiolysis, we have deduced reaction mechanisms for LDL peroxidation initiated by O2-·/HO2· free radicals.

引用

页码：228 / 236

页数：9

共 33 条

[1]

ALLAIN CC, 1974, CLIN CHEM, V20, P470

[2]

ARUDI RL, 1983, OXYRADICALS THEIR SC, V1, P26

[3]

AVOGARO P, 1988, OXYGEN RADICALS BIOL, P391

[4] ACID DISSOCIATION CONSTANT AND DECAY KINETICS OF PERHYDROXYL RADICAL [J].

BEHAR, D ;

CZAPSKI, G ;

RABANI, J ;

DORFMAN, LM ;

SCHWARZ, HA .

JOURNAL OF PHYSICAL CHEMISTRY, 1970, 74 (17) :3209-&

[5]

BIELSKI BHJ, 1983, J BIOL CHEM, V258, P4759

[6] LIPOPROTEIN METABOLISM IN THE MACROPHAGE - IMPLICATIONS FOR CHOLESTEROL DEPOSITION IN ATHEROSCLEROSIS [J].

BROWN, MS ;

GOLDSTEIN, JL .

ANNUAL REVIEW OF BIOCHEMISTRY, 1983, 52 :223-261

[7] MONOCYTES AND NEUTROPHILS OXIDIZE LOW-DENSITY LIPOPROTEIN MAKING IT CYTO-TOXIC [J].

CATHCART, MK ;

MOREL, DW ;

CHISOLM, GM .

JOURNAL OF LEUKOCYTE BIOLOGY, 1985, 38 (02) :341-350

[8] THE ACTION OF HYDROGEN-PEROXIDE ON THE FORMATION OF THIOBARBITURIC ACID-REACTIVE MATERIAL FROM MICROSOMES, LIPOSOMES OR FROM DNA DAMAGED BY BLEOMYCIN OR PHENANTHROLINE - ARTIFACTS IN THE THIOBARBITURIC ACID TEST [J].

CECCHINI, R ;

ARUOMA, OI ;

HALLIWELL, B .

FREE RADICAL RESEARCH COMMUNICATIONS, 1990, 10 (4-5) :245-258

[9] HISTIDINE AND PROLINE ARE IMPORTANT SITES OF FREE-RADICAL DAMAGE TO PROTEINS [J].

DEAN, RT ;

WOLFF, SP ;

MCELLIGOTT, MA .

FREE RADICAL RESEARCH COMMUNICATIONS, 1989, 7 (02) :97-103

[10] EINE NEUE BESTIMMUNG DER NEUTRALFETTE IM BLUTSERUM UND GEWEBE .I. PRINZIP DURCHFUHRUNG UND BESPRECHUNG DER METHODE [J].

EGGSTEIN, M ;

KREUTZ, FH .

KLINISCHE WOCHENSCHRIFT, 1966, 44 (05) :262-&

← 1 2 3 4 →