BCL-2 GENE IS HIGHLY EXPRESSED DURING NEUROGENESIS IN THE CENTRAL-NERVOUS-SYSTEM

被引：95

作者：

ABEDOHMAE, S ^{[1
]}

HARADA, N ^{[1
]}

YAMADA, K ^{[1
]}

TANAKA, R ^{[1
]}

机构：

[1] FUJITA HLTH UNIV, INST COMPREHENS MED SCI, DIV MOLEC GENET, TOYOAKE, AICHI 47011, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 191卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.1304

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An analytical method for quantitation of the RNA transcripts of murine bcl-2 gene was developed. The PCR products from bcl-2α and bcl-2β mRNA were fluorometrically analyzed and their specific contents were calculated by the internal standard method. Both bcl-2 mRNAs in adult mice were transcribed at the highest level in the thymus and at a comparable level in the spleen. Aside from the immune system, the brain gave the most abundant levels of the bcl-2 mRNAs. The ratios of bcl-2β mRNA to bcl-2α mRNA in the thymus and spleen were significantly higher than those in other tissues. During development of the brain, the bcl-2α and bcl-2β mRNA levels were highest on embryonic day 15, and about two and three times higher than those of adult, respectively. The results suggest that the bcl-2 gene functions to regulate development and survival of neurons in the central nervous system. © 1993 Academic Press, Inc.

引用

页码：915 / 921

页数：7

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