A CHICKEN RAD51 HOMOLOG IS EXPRESSED AT HIGH-LEVELS IN LYMPHOID AND REPRODUCTIVE-ORGANS

被引：110

作者：

BEZZUBOVA, O

SHINOHARA, A

MUELLER, RG

OGAWA, H

BUERSTEDDE, JM

机构：

[1] BASEL INST IMMUNOL,GRENZACHERSTR 487,CH-4005 BASEL,SWITZERLAND

[2] OSAKA UNIV,FAC SCI,DEPT BIOL,TOYONAKA,OSAKA 560,JAPAN

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 07期

关键词：

D O I：

10.1093/nar/21.7.1577

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Comparisons of the amino acid sequences of three yeast RecA-like proteins, Rad51 and DMC1 from S.cereviseae and Rad51 from S.pombe, revealed several highly conserved regions. Degenerated oligonucleotides encoding two of these regions were used for the polymerase chain reaction to clone a chicken RecA-like gene. The encoded protein shares 68% and 49% identical amino acids with the Rad51 and DMC1 proteins. The strong sequence conservation between the yeast and chicken genes indicates that RecA homologues are conserved throughout evolution from prokaryots to higher eukaryotes. High expression of the chicken Rad51 gene was found within the organs of lymphoid and germ cell development suggesting its involvement in lymphoid and meiotic recombination.

引用

页码：1577 / 1580

页数：4

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[11] POINT MUTATIONS DEFINE A SEQUENCE FLANKING THE AUG INITIATOR CODON THAT MODULATES TRANSLATION BY EUKARYOTIC RIBOSOMES [J].