THE COMPLEXITY OF THE RAB AND RHO GTP-BINDING PROTEIN SUBFAMILIES REVEALED BY A PCR CLONING APPROACH

被引：108

作者：

CHAVRIER, P ^{[1
]}

SIMONS, K ^{[1
]}

ZERIAL, M ^{[1
]}

机构：

[1] EUROPEAN MOLEC BIOL LAB,CELL BIOL PROGRAM,POSTFACH 102209,W-6900 HEIDELBERG,GERMANY

来源：

GENE | 1992年 / 112卷 / 02期

关键词：

LOW-MOLECULAR-WEIGHT GTP-BINDING PROTEINS; POLYMERASE CHAIN REACTION; DEGENERATE OLIGODEOXYRIBONUCLEOTIDES; MOUSE;

D O I：

10.1016/0378-1119(92)90387-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Partial sequences corresponding to eleven novel Rab proteins and one new Rho protein have been isolated using a PCR-based cloning approach. These results confirm that the overall diversity of the Rab and Rho protein subfamilies account for more than thirty different members in mammalian cells.

引用

页码：261 / 264

页数：4

共 16 条

[1] DO GTPASES DIRECT MEMBRANE TRAFFIC IN SECRETION [J].

BOURNE, HR .

CELL, 1988, 53 (05) :669-671

[2] LOCALIZATION OF LOW-MOLECULAR-WEIGHT GTP BINDING-PROTEINS TO EXOCYTIC AND ENDOCYTIC COMPARTMENTS [J].

CHAVRIER, P ;

PARTON, RG ;

HAURI, HP ;

SIMONS, K ;

ZERIAL, M .

CELL, 1990, 62 (02) :317-329

[3] MOLECULAR-CLONING OF YPT1/SEC4-RELATED CDNAS FROM AN EPITHELIAL-CELL LINE [J].

CHAVRIER, P ;

VINGRON, M ;

SANDER, C ;

SIMONS, K ;

ZERIAL, M .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (12) :6578-6585

[4] CHARACTERIZATION OF 4 NOVEL RAS-LIKE GENES EXPRESSED IN A HUMAN TERATOCARCINOMA CELL-LINE [J].

DRIVAS, GT ;

SHIH, A ;

COUTAVAS, E ;

RUSH, MG ;

DEUSTACHIO, P .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (04) :1793-1798

[5] RAPID PRODUCTION OF FULL-LENGTH CDNAS FROM RARE TRANSCRIPTS - AMPLIFICATION USING A SINGLE GENE-SPECIFIC OLIGONUCLEOTIDE PRIMER [J].

FROHMAN, MA ;

DUSH, MK ;

MARTIN, GR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :8998-9002

[6] A YEAST GENE ENCODING A PROTEIN HOMOLOGOUS TO THE HUMAN C-HAS/BAS PROTO-ONCOGENE PRODUCT [J].

GALLWITZ, D ;

DONATH, C ;

SANDER, C .

NATURE, 1983, 306 (5944) :704-707

[7] RAB5 CONTROLS EARLY ENDOSOME FUSION INVITRO [J].

GORVEL, JP ;

CHAVRIER, P ;

ZERIAL, M ;

GRUENBERG, J .

CELL, 1991, 64 (05) :915-925

[8] MOLECULAR CHARACTERIZATION OF CDC42, A SACCHAROMYCES-CEREVISIAE GENE INVOLVED IN THE DEVELOPMENT OF CELL POLARITY [J].

JOHNSON, DI ;

PRINGLE, JR .

JOURNAL OF CELL BIOLOGY, 1990, 111 (01) :143-152

[9]

MATSUI Y, 1988, J BIOL CHEM, V263, P11071

[10] STRUCTURE OF THE GUANINE-NUCLEOTIDE-BINDING DOMAIN OF THE HA-RAS ONCOGENE PRODUCT P21 IN THE TRIPHOSPHATE CONFORMATION [J].

PAI, EF ;

KABSCH, W ;

KRENGEL, U ;

HOLMES, KC ;

JOHN, J ;

WITTINGHOFER, A .

NATURE, 1989, 341 (6239) :209-214

← 1 2 →