MOLECULAR-CLONING OF YPT1/SEC4-RELATED CDNAS FROM AN EPITHELIAL-CELL LINE

被引：207

作者：

CHAVRIER, P ^{[1
]}

VINGRON, M ^{[1
]}

SANDER, C ^{[1
]}

SIMONS, K ^{[1
]}

ZERIAL, M ^{[1
]}

机构：

[1] EUROPEAN MOLEC BIOL LAB,POSTFACH 102209,MEYERHOFSTR 1,W-6900 HEIDELBERG,GERMANY

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 12期

关键词：

D O I：

10.1128/MCB.10.12.6578

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Molecular analysis of Saccharomyces cerevisiae secretion mutants has led to the identification of two Ras-like GTP-binding proteins, Ypt1p and Sec4p, which are essential for transport along the exocytic route. To study the regulation of membrane traffic in epithelial cells, a set of 11 clones encoding proteins similar to the YPT1/SEC4 products were isolated from an MDCK (Madin-Darby canine kidney) cell cDNA library. Four of these proteins, Rab8, -9, -10, and -11, are novel members of this subfamily of Ras-like proteins, and two of them are closely related to Ypt1p and Sec4p. The ratio of the number of clones isolated over the total number screened reveals a high level of complexity for this subfamily of GTP-binding proteins. This diversity supports their proposed function in controlling different steps in membrane traffic.

引用

页码：6578 / 6585

页数：8

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