IDENTIFICATION OF ALPHA-2-SUBUNIT AND ALPHA-3-SUBUNIT OF THE GABA-A-BENZODIAZEPINE RECEPTOR COMPLEX PURIFIED FROM THE BRAINS OF YOUNG-RATS

被引：51

作者：

FUCHS, K

ADAMIKER, D

SIEGHART, W

机构：

[1] UNIV VIENNA,PSYCHIAT KLIN,DEPT BIOCHEM PSYCHIAT,WAHRINGER GURTEL 18-20,A-1090 VIENNA,AUSTRIA

[2] RES INST LAB ANIM BREEDING,HIMBERG,AUSTRIA

来源：

FEBS LETTERS | 1990年 / 261卷 / 01期

关键词：

Antibody; GABA[!sub]A[!/sub]-benzodiazepine receptor; Heterogeneity; Subunit;

D O I：

10.1016/0014-5793(90)80634-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polyclonal antibodies were raised to synthetic amino acid sequences of the bovine GABAA receptor α2- and α3-subunits and purified by affinity chromatography on a column coupled with the respective peptide. Anti-peptide α2(416-424) and anti-peptide α3(459-467) antibodies immunoprecipitated GABAA receptors and recognized a protein of 53 kDa (P53) and 59 kDa (P59), respectively, in Western blots of GABAA receptors purified from the brains of 5-10 day old rats. P53 as well as P59 are specifically photolabeled by [3H]flunitrazepam and are recognized by the α-subunit specific monoclonal antibody bd 28. © 1990.

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页码：52 / 54

页数：3

相关论文

共 17 条

[1] A RAPID AND SIMPLE METHOD FOR EFFICIENT COATING OF MICROTITER PLATES USING LOW AMOUNTS OF ANTIGEN IN THE PRESENCE OF DETERGENT [J].

DREXLER, G ;

EICHINGER, A ;

WOLF, C ;

SIEGHART, W .

JOURNAL OF IMMUNOLOGICAL METHODS, 1986, 95 (01) :117-122

[2] POSTNATAL-DEVELOPMENT OF PROTEINS ASSOCIATED WITH DIFFERENT BENZODIAZEPINE RECEPTORS [J].

EICHINGER, A ;

SIEGHART, W .

JOURNAL OF NEUROCHEMISTRY, 1986, 46 (01) :173-180

[3] EVIDENCE FOR THE EXISTENCE OF SEVERAL DIFFERENT ALPHA-SUBUNIT AND BETA-SUBUNIT OF THE GABA BENZODIAZEPINE RECEPTOR COMPLEX FROM RAT-BRAIN [J].

FUCHS, K ;

SIEGHART, W .

NEUROSCIENCE LETTERS, 1989, 97 (03) :329-333

[4] IMMUNOGENIC STRUCTURE OF THE INFLUENZA-VIRUS HEMAGGLUTININ [J].

GREEN, N ;

ALEXANDER, H ;

OLSON, A ;

ALEXANDER, S ;

SHINNICK, TM ;

SUTCLIFFE, JG ;

LERNER, RA .

CELL, 1982, 28 (03) :477-487

[5] MONOCLONAL-ANTIBODIES REVEAL STRUCTURAL HOMOGENEITY OF GAMMA-AMINOBUTYRIC ACID BENZODIAZEPINE RECEPTORS IN DIFFERENT BRAIN-AREAS [J].

HARING, P ;

STAHLI, C ;

SCHOCH, P ;

TAKACS, B ;

STAEHELIN, T ;

MOHLER, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (14) :4837-4841

[6]

Kessler S W, 1981, Methods Enzymol, V73, P442

[7] STRUCTURAL AND FUNCTIONAL BASIS FOR GABAA RECEPTOR HETEROGENEITY [J].

LEVITAN, ES ;

SCHOFIELD, PR ;

BURT, DR ;

RHEE, LM ;

WISDEN, W ;

KOHLER, M ;

FUJITA, N ;

RODRIGUEZ, HF ;

STEPHENSON, A ;

DARLISON, MG ;

BARNARD, EA ;

SEEBURG, PH .

NATURE, 1988, 335 (6185) :76-79

[8] EPITOPE MAPPING EMPLOYING ANTIBODIES RAISED AGAINST SHORT SYNTHETIC PEPTIDES - A STUDY OF THE NICOTINIC ACETYLCHOLINE-RECEPTOR [J].

MAELICKE, A ;

PLUMERWILK, R ;

FELS, G ;

SPENCER, SR ;

ENGELHARD, M ;

VELTEL, D ;

CONTITRONCONI, BM .

BIOCHEMISTRY, 1989, 28 (03) :1396-1405

[9] TYPE-I AND TYPE-II GABAA-BENZODIAZEPINE RECEPTORS PRODUCED IN TRANSFECTED CELLS [J].

PRITCHETT, DB ;

LUDDENS, H ;

SEEBURG, PH .

SCIENCE, 1989, 245 (4924) :1389-1392

[10] IMPORTANCE OF A NOVEL GABAA RECEPTOR SUBUNIT FOR BENZODIAZEPINE PHARMACOLOGY [J].

PRITCHETT, DB ;

SONTHEIMER, H ;

SHIVERS, BD ;

YMER, S ;

KETTENMANN, H ;

SCHOFIELD, PR ;

SEEBURG, PH .

NATURE, 1989, 338 (6216) :582-585