PURIFICATION, CLONING, EXPRESSION AND BIOLOGICAL CHARACTERIZATION OF AN INTERLEUKIN-1 RECEPTOR ANTAGONIST PROTEIN

被引：670

作者：

CARTER, DB

DEIBEL, MR

DUNN, CJ

TOMICH, CSC

LABORDE, AL

SLIGHTOM, JL

BERGER, AE

BIENKOWSKI, MJ

SUN, FF

MCEWAN, RN

HARRIS, PKW

YEM, AW

WASZAK, GA

CHOSAY, JG

SIEU, LC

HARDEE, MM

ZURCHERNEELY, HA

REARDON, IM

HEINRIKSON, RL

TRUESDELL, SE

SHELLY, JA

EESSALU, TE

TAYLOR, BM

TRACEY, DE

机构：

[1] UPJOHN CO,DEPT BIOPOLYMER CHEM,KALAMAZOO,MI 49007

[2] UPJOHN CO,DEPT HYPERSENSIT DIS RES,KALAMAZOO,MI 49007

[3] UPJOHN CO,DEPT CHEM & BIOL SCREENING,KALAMAZOO,MI 49007

[4] UPJOHN CO,DEPT CELL BIOL,KALAMAZOO,MI 49007

来源：

NATURE | 1990年 / 344卷 / 6267期

关键词：

D O I：

10.1038/344633a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A human myelomonocytic cell line, U937, produced an interleukin-1 (IL-1) receptor antagonist protein (IRAP) which was purified and partially sequenced. A complementary DNA coding for IRAP was cloned and sequenced. The mature translation product of the cDNA has been expressed in Escherichia coli and was an active competitive inhibitor of the binding of IL-1 to the T-cell/fibroblast form of the IL-1 receptor. Recombinant IRAP specifically inhibited IL-1 bioactivity on T cells and endothelial cells in vitro and was a potent inhibitor of IL-1 induced corticosterone production in vivo. © 1990 Nature Publishing Group.

引用

页码：633 / 638

页数：6

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