MORPHOLOGICAL AND CYTOSKELETAL CHANGES IN EPITHELIAL-CELLS OCCUR IMMEDIATELY UPON INTERACTION WITH SALMONELLA-TYPHIMURIUM GROWN UNDER LOW-OXYGEN CONDITIONS

被引：172

作者：

FRANCIS, CL ^{[1
]}

STARNBACH, MN ^{[1
]}

FALKOW, S ^{[1
]}

机构：

[1] NIAID, ROCKY MT LAB, HAMILTON, MT 59840 USA

来源：

MOLECULAR MICROBIOLOGY | 1992年 / 6卷 / 21期

关键词：

D O I：

10.1111/j.1365-2958.1992.tb01765.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Salmonella typhimurium grown under oxygen-limiting conditions were found to enter into, elicit actin filament rearrangement in, and effect morphological changes upon HEp-2 cells within 15 min after infection. Video microscopy revealed that host cell morphological changes associated with entry began within 1 min of productive adherence. Polarized Caco-2 cell morphology was affected 40 s after infection with low-oxygen-grown S. typhimurium. Stationary-phase S. typhimurium did not elicit these phenomena within this time-period even when adherence was enhanced with the afimbrial adhesin, AFA-I. Thus, environmental cues regulate S. typhimurium invasion factors, allowing for immediate entry into host cells. Additionally, actin filament rearrangement and morphological changes in the eukaryotic host cell are essential for entry and occur within minutes of infection.

引用

页码：3077 / 3087

页数：11

共 32 条

[11] COMPARISON OF THE INVASION STRATEGIES USED BY SALMONELLA-CHOLERAE-SUIS, SHIGELLA-FLEXNERI AND YERSINIA-ENTEROCOLITICA TO ENTER CULTURED ANIMAL-CELLS - ENDOSOME ACIDIFICATION IS NOT REQUIRED FOR BACTERIAL INVASION OR INTRACELLULAR REPLICATION [J].