PROPERTIES OF PRIMER-DEPENDENT STARCH SYNTHESIS CATALYZED BY STARCH SYNTHASE FROM POTATO-TUBERS

被引：16

作者：

BABA, T ^{[1
]}

NORO, M ^{[1
]}

HIROTO, M ^{[1
]}

ARAI, Y ^{[1
]}

机构：

[1] TOINGAKUEN TECH COLL,KANAGAWA 227,JAPAN

来源：

PHYTOCHEMISTRY | 1990年 / 29卷 / 03期

关键词：

ADP-Glc; potato tubers; purification; Solanum tuberosum; Solonaceae; starch biosynthesis; starch synthase;

D O I：

10.1016/0031-9422(90)80007-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A starch synthase was partially purified from soluble extracts of potato tubers by successive chromatographic separation on maltotriose-bovine serum albumin conjugated to Sepharose, DEAE-Toyopearl 650-S and Sepharose CL-6B columns. The enzyme activity was completely dependent on added primer. Enzyme activity increased with increasing concentrations of sodium citrate, but sodium citrate was not absolutely necessary for the activity. Rabbit liver glycogen was the best primer for the enzyme, whereas malto-oligosaccharides were not effective. The enzyme elongated only α-1,4-linked chain by transferring glucose from ADPGlc into the primer. Thus, this particular starch synthase is different from already known enzymes of this type in several respects, notably its primer requirement, its specificity toward primers, and the stimulation of the primed reaction with sodium citrate. © 1990.

引用

页码：719 / 723

页数：5

共 26 条

[1] ROLE OF THE RECESSIVE AMYLOSE-EXTENDER ALLELE IN STARCH BIOSYNTHESIS OF MAIZE .2. BRANCHING ENZYME FROM AMYLOMAIZE ENDOSPERMS [J].