CELL CYCLE-DEPENDENT SPECIFIC POSITIONING AND CLUSTERING OF CENTROMERES AND TELOMERES IN FISSION YEAST

被引:453
作者
FUNABIKI, H
HAGAN, I
UZAWA, S
YANAGIDA, M
机构
[1] Biochem./Molecular Biology Dept., School of Biological Sciences, University of Manchester, Manchester M13 9PT, Oxford Road
关键词
D O I
10.1083/jcb.121.5.961
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Fluorescence in situ hybridization (FISH) shows that fission yeast centromeres and telomeres make up specific spatial arrangements in the nucleus. Their positioning and clustering are cell cycle regulated. In G2, centromeres cluster adjacent to the spindle pole body (SPB), while in mitosis, their association with each other and with the SPB is disrupted. Similarly, telomeres cluster at the nuclear periphery in G2 and their associations are disrupted in mitosis. Mitotic centromeres interact with the spindle. They remain undivided until the spindle reaches a critical length, then separate and move towards the poles. This demonstrated, for the first time, that anaphase A occurs in fission yeast. The mode of anaphase A and B is similar to that of higher eukaryotes. In nda3 and cut7 mutants defective in tubulin or a kinesin-related motor, cells are blocked in early stages of mitosis due to the absence of the spindle, and centromeres dissociate but remain close to the SPB, whereas in a metaphase-arrested nuc2 mutant, they reside at the middle of the spindle. FISH is therefore a powerful tool for analyzing mitotic chromosome movement and disjunction using various mutants. Surprisingly, in top2 defective in DNA topoisomerase II, while most chromatid DNAs remain undivided, sister centromeres are separated. Significance of this finding is discussed. In contrast, most chromatid DNAs are separated but telomeric DNAs are not in cut1 mutant. In cut1, the dependence of SPB duplication on the completion of mitosis is abolished. In crm1 mutant cells defective in higher-order chromosome organization, the interphase arrangements of centromeres and telomeres are disrupted.
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页码:961 / 976
页数:16
相关论文
共 72 条
[21]   NOVEL POTENTIAL MITOTIC MOTOR PROTEIN ENCODED BY THE FISSION YEAST CUT7+ GENE [J].
HAGAN, I ;
YANAGIDA, M .
NATURE, 1990, 347 (6293) :563-566
[22]   INTRAMITOTIC CONTROLS IN THE FISSION YEAST SCHIZOSACCHAROMYCES-POMBE - THE EFFECT OF CELL-SIZE ON SPINDLE LENGTH AND THE TIMING OF MITOTIC EVENTS [J].
HAGAN, IM ;
RIDDLE, PN ;
HYAMS, JS .
JOURNAL OF CELL BIOLOGY, 1990, 110 (05) :1617-1621
[23]  
HAGAN IM, 1988, J CELL SCI, V89, P343
[24]   IDENTIFICATION OF DNA REGIONS REQUIRED FOR MITOTIC AND MEIOTIC FUNCTIONS WITHIN THE CENTROMERE OF SCHIZOSACCHAROMYCES-POMBE CHROMOSOME-I [J].
HAHNENBERGER, KM ;
CARBON, J ;
CLARKE, L .
MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (04) :2206-2215
[25]   CONSTRUCTION OF FUNCTIONAL ARTIFICIAL MINICHROMOSOMES IN THE FISSION YEAST SCHIZOSACCHAROMYCES-POMBE [J].
HAHNENBERGER, KM ;
BAUM, MP ;
POLIZZI, CM ;
CARBON, J ;
CLARKE, L .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (02) :577-581
[26]   BEHAVIOR OF KINETOCHORES DURING MITOSIS IN THE FUNGUS SAPROLEGNIA-FERAX [J].
HEATH, IB .
JOURNAL OF CELL BIOLOGY, 1980, 84 (03) :531-546
[27]   THE ARRANGEMENT OF INTERPHASE CHROMOSOMES - STRUCTURAL AND FUNCTIONAL-ASPECTS [J].
HILLIKER, AJ ;
APPELS, R .
EXPERIMENTAL CELL RESEARCH, 1989, 185 (02) :297-318
[28]   SNAP HELIX WITH KNOB AND HOLE - ESSENTIAL REPEATS IN S-POMBE NUCLEAR-PROTEIN NUC2+ [J].
HIRANO, T ;
KINOSHITA, N ;
MORIKAWA, K ;
YANAGIDA, M .
CELL, 1990, 60 (02) :319-328
[29]   A TEMPERATURE-SENSITIVE MUTATION OF THE SCHIZOSACCHAROMYCES-POMBE GENE NUC2+THAT ENCODES A NUCLEAR SCAFFOLD-LIKE PROTEIN BLOCKS SPINDLE ELONGATION IN MITOTIC ANAPHASE [J].
HIRANO, T ;
HIRAOKA, Y ;
YANAGIDA, M .
JOURNAL OF CELL BIOLOGY, 1988, 106 (04) :1171-1183
[30]   ISOLATION AND CHARACTERIZATION OF SCHIZOSACCHAROMYCES-POMBE CUT MUTANTS THAT BLOCK NUCLEAR DIVISION BUT NOT CYTOKINESIS [J].
HIRANO, T ;
FUNAHASHI, S ;
UEMURA, T ;
YANAGIDA, M .
EMBO JOURNAL, 1986, 5 (11) :2973-2979