Detection of Leishmania in the blood of early kala-azar patients with the aid of the polymerase chain reaction

被引：56

作者：

Adhya, S ^{[1
]}

Chatterjee, M ^{[1
]}

Hassan, MQ ^{[1
]}

Mukherjee, S ^{[1
]}

Sen, S ^{[1
]}

机构：

[1] SEN MED RES CTR,PATNA 800001,BIHAR,INDIA

来源：

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE | 1995年 / 89卷 / 06期

关键词：

visceral leishmaniasis; Leishmania donovani; diagnosis; ELISA; PCR; microscopy; India;

D O I：

10.1016/0035-9203(95)90416-6

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Samples from 39 patients with symptoms suggestive of early visceral leishmaniasis were independently assayed by microscopy of tissue smears, enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) of blood deoxyribonucleic acid. Of these patients, 19 were confirmed as positive or negative by all 3 tests; 11 patients (28%) negative by smear were positive by ELISA and PCR; and 7 (18%) were positive by PCR alone. These results demonstrate the high sensitivity of the nbn-invasive PCR and, to a smaller extent, ELISA, in the early diagnosis of visceral leishmaniasis.

引用

页码：622 / 624

页数：3

共 14 条

[11] PRIMER-DIRECTED ENZYMATIC AMPLIFICATION OF DNA WITH A THERMOSTABLE DNA-POLYMERASE [J].