A NEW FAMILY OF SUGAR-INDUCIBLE EXPRESSION VECTORS FOR ESCHERICHIA-COLI

被引：58

作者：

CAGNON, C ^{[1
]}

VALVERDE, V ^{[1
]}

MASSON, JM ^{[1
]}

机构：

[1] CNRS,CTR TRANSFERT BIOTECHNOL MICROBIOL,INST NATL SCI APPL,URA 544,F-31077 TOULOUSE,FRANCE

来源：

PROTEIN ENGINEERING | 1991年 / 4卷 / 07期

关键词：

ARABINOSE PROMOTOR; BLEOMYCIN BINDING PROTEIN; BETA-GALACTOSIDASE ASSAY; SECRETION VECTORS;

D O I：

10.1093/protein/4.7.843

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A set of 11 expression vectors was constructed, each of them harbouring a cloning cassette under the control of the araB promoter. Some of these vectors enable expression of foreign proteins in the cytoplasm, while others include a synthetic sequence coding for a very efficient secretion signal sequence. Other features are an f1 origin of replication (in plus or minus orientation) and a promoter(up) mutation that enhances the already very high level of expression from these vectors. With such a versatile vector family, cloning, sequencing and site-directed mutagenesis can be performed on the same vector, and the level of expression can be defined according to the specific constraints of a given protein.

引用

页码：843 / 847

页数：5

共 39 条

[1] ATG VECTORS FOR REGULATED HIGH-LEVEL EXPRESSION OF CLONED GENES IN ESCHERICHIA-COLI [J].

AMANN, E ;

BROSIUS, J .

GENE, 1985, 40 (2-3) :183-190

[2] PRODUCTION OF A FULL LENGTH TAT PROTEIN IN ESCHERICHIA-COLI AND ITS PURIFICATION [J].

ARMENGAUD, J ;

PEREZ, LD ;

LEMAY, P ;

MASSON, JM .

FEBS LETTERS, 1991, 282 (01) :157-160

[3] ESCHERICHIA-COLI SECRETION OF AN ACTIVE CHIMERIC ANTIBODY FRAGMENT [J].

BETTER, M ;

CHANG, CP ;

ROBINSON, RR ;

HORWITZ, AH .

SCIENCE, 1988, 240 (4855) :1041-1043

[4] PROTEIN SECRETION CONTROLLED BY A SYNTHETIC GENE IN ESCHERICHIA-COLI [J].

BLANCHINROLAND, S ;

MASSON, JM .

PROTEIN ENGINEERING, 1989, 2 (06) :473-480

[5] TOXICITY OF AN OVERPRODUCED FOREIGN GENE-PRODUCT IN ESCHERICHIA-COLI AND ITS USE IN PLASMID VECTORS FOR THE SELECTION OF TRANSCRIPTION TERMINATORS [J].

BROSIUS, J .

GENE, 1984, 27 (02) :161-172

[6]

BROSIUS J, 1982, J BIOL CHEM, V257, P9205

[7] FUSION OF ESCHERICHIA-COLI LAC GENES TO ARA PROMOTER - GENERAL TECHNIQUE USING BACTERIOPHAGE MU-1 INSERTIONS [J].

CASADABAN, MJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (03) :809-813

[8] ANALYSIS OF GENE-CONTROL SIGNALS BY DNA-FUSION AND CLONING IN ESCHERICHIA-COLI [J].

CASADABAN, MJ ;

COHEN, SN .

JOURNAL OF MOLECULAR BIOLOGY, 1980, 138 (02) :179-207

[9]

DECLERCK N, 1990, J BIOL CHEM, V265, P15481

[10] PEMBL - A NEW FAMILY OF SINGLE STRANDED PLASMIDS [J].

DENTE, L ;

CESARENI, G ;

CORTESE, R .

NUCLEIC ACIDS RESEARCH, 1983, 11 (06) :1645-1655

← 1 2 3 4 →