PRODUCTION OF A FULL LENGTH TAT PROTEIN IN ESCHERICHIA-COLI AND ITS PURIFICATION

被引：6

作者：

ARMENGAUD, J ^{[1
]}

PEREZ, LD ^{[1
]}

LEMAY, P ^{[1
]}

MASSON, JM ^{[1
]}

机构：

[1] INST NATL SCI APPL,CTR TRANSFERT BIOTECHNOL & MICROBIOL,COMPLEXE SCI RANGUEIL,F-31077 TOULOUSE,FRANCE

来源：

FEBS LETTERS | 1991年 / 282卷 / 01期

关键词：

HIV; TAT; REGULATORY PROTEIN; GENE EXPRESSION; PURIFICATION;

D O I：

10.1016/0014-5793(91)80467-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A full length tat gene was constructed by a combination of polymerase chain reaction (PCR) for the first exon and chemical synthesis for the second exon. This gene was expressed in E. coli under the control of the strongly regulated araB promoter, either directly or fused to a secretion signal encoding sequence. We then defined a rapid, three-step procedure for the purification of the Tat protein.

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页码：157 / 160

页数：4

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