EXACT CLEAVAGE SITE OF ALZHEIMER AMYLOID PRECURSOR IN NEURONAL PC-12 CELLS

被引：202

作者：

ANDERSON, JP

ESCH, FS

KEIM, PS

SAMBAMURTI, K

LIEBERBURG, I

ROBAKIS, NK

机构：

[1] MT SINAI MED CTR,DEPT PSYCHIAT,BOX 1229,1 GUSTAVE LEVY PL,NEW YORK,NY 10029

[2] ATHENA NEUROSCI INC,S SAN FRANCISCO,CA

[3] MT SINAI MED CTR,FISHBERG RES CTR NEUROBIOL,NEW YORK,NY 10029

来源：

NEUROSCIENCE LETTERS | 1991年 / 128卷 / 01期

关键词：

ALZHEIMER DISEASE; ALZHEIMER AMYLOID PRECURSOR PROTEIN; SECRETORY PROTEASE; PC-12; CELL;

D O I：

10.1016/0304-3940(91)90775-O

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We have identified the secretory cleavage site in the Alzheimer amyloid precursor (APP) in a non-transfected neuronal cell line, using cyanogen bromide digests of APP purified from medium conditioned by PC-12 cells which were differentiated to a neuronal phenotype. The results obtained are most consistent with proteolysis of the Lys16-Leu17 bond in the beta-amyloid peptide, followed by partial removal of Lys16 by a basic carboxypeptidase.

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页码：126 / 128

页数：3

相关论文

共 19 条

[11] A NEW A4-AMYLOID MESSENGER-RNA CONTAINS A DOMAIN HOMOLOGOUS TO SERINE PROTEINASE-INHIBITORS [J].

PONTE, P ;

GONZALEZDEWHITT, P ;

SCHILLING, J ;

MILLER, J ;

HSU, D ;

GREENBERG, B ;

DAVIS, K ;

WALLACE, W ;

LIEBERBURG, I ;

FULLER, F ;

CORDELL, B .

NATURE, 1988, 331 (6156) :525-527

[12] NERVE AND EPIDERMAL GROWTH-FACTORS INDUCE THE RELEASE OF THE ALZHEIMER AMYLOID PRECURSOR FROM PC-12 CELL-CULTURES [J].

REFOLO, LM ;

SALTON, SRJ ;

ANDERSON, JP ;

MEHTA, P ;

ROBAKIS, NK .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 164 (02) :664-670

[13] MOLECULAR-CLONING AND CHARACTERIZATION OF A CDNA-ENCODING THE CEREBROVASCULAR AND THE NEURITIC PLAQUE AMYLOID PEPTIDES [J].

ROBAKIS, NK ;

RAMAKRISHNA, N ;

WOLFE, G ;

WISNIEWSKI, HM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (12) :4190-4194

[14] THE REGULATION OF AMYLOID-BETA-PROTEIN PRECURSOR SECRETION AND ITS MODULATORY ROLE IN CELL-ADHESION [J].

SCHUBERT, D ;

JIN, LW ;

SAITOH, T ;

COLE, G .

NEURON, 1989, 3 (06) :689-694

[15] EVIDENCE THAT BETA-AMYLOID PROTEIN IN ALZHEIMERS-DISEASE IS NOT DERIVED BY NORMAL PROCESSING [J].

SISODIA, SS ;

KOO, EH ;

BEYREUTHER, K ;

UNTERBECK, A ;

PRICE, DL .

SCIENCE, 1990, 248 (4954) :492-495

[16] AMYLOID BETA-PROTEIN GENE - CDNA, MESSENGER-RNA DISTRIBUTION, AND GENETIC-LINKAGE NEAR THE ALZHEIMER LOCUS [J].

TANZI, RE ;

GUSELLA, JF ;

WATKINS, PC ;

BRUNS, GAP ;

STGEORGEHYSLOP, P ;

VANKEUREN, ML ;

PATTERSON, D ;

PAGAN, S ;

KURNIT, DM ;

NEVE, RL .

SCIENCE, 1987, 235 (4791) :880-884

[17] PROTEASE INHIBITOR DOMAIN ENCODED BY AN AMYLOID PROTEIN-PRECURSOR MESSENGER-RNA ASSOCIATED WITH ALZHEIMERS-DISEASE [J].

TANZI, RE ;

MCCLATCHEY, AI ;

LAMPERTI, ED ;

VILLAKOMAROFF, L ;

GUSELLA, JF ;

NEVE, RL .

NATURE, 1988, 331 (6156) :528-530

[18]

VANNOSTRAND WE, 1989, NATURE, V341, P546

[19] IDENTIFICATION, BIOGENESIS, AND LOCALIZATION OF PRECURSORS OF ALZHEIMERS-DISEASE A4 AMYLOID PROTEIN [J].

WEIDEMANN, A ;

KONIG, G ;

BUNKE, D ;

FISCHER, P ;

SALBAUM, JM ;

MASTERS, CL ;

BEYREUTHER, K .

CELL, 1989, 57 (01) :115-126