NERVE GROWTH-FACTOR INDUCES SENSITIVITY TO BOTULINUM NEUROTOXIN TYPE-A IN NOREPINEPHRINE-SECRETING PC12 CELLS

被引：13

作者：

BANERJEE, A

MARTIN, TFJ

DASGUPTA, BR

机构：

[1] UNIV WISCONSIN,DEPT FOOD MICROBIOL & TOXICOL,MADISON,WI 53706

[2] UNIV WISCONSIN,DEPT ZOOL,MADISON,WI 53706

来源：

NEUROSCIENCE LETTERS | 1993年 / 164卷 / 1-2期

关键词：

BOTULINUM NEUROTOXIN; NERVE GROWTH FACTOR; PC12; CELL; NOREPINEPHRINE RELEASE;

D O I：

10.1016/0304-3940(93)90865-I

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Inhibition of Ca2+-activated norepinephrine secretion by the botulinum neurotoxin (NT) serotypes A and E was examined in permeabilized PC12 cells. The dichain type E NT reduced with dithiothreitol (DTT) completely inhibited secretion whereas the dichain type A NT reduced with DTT exhibited incomplete inhibitory activity. In contrast, Ca2+-activated secretion in PC12 cells treated with nerve growth factor (NGF) was completely inhibited by reduced type A NT. The NGF-treated PC12 cells retained a sensitivity to the type E NT similar to that of untreated PC12 cells. These results indicate that the intracellular mechanisms of inhibition of the types E and A NTs are distinct. NGF appears to either induce the expression of a component selectively required for type A NT sensitivity, or otherwise modifies the secretory apparatus to acquire type A NT sensitivity.

引用

页码：93 / 96

页数：4

共 26 条

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[22] INHIBITION OF CALCIUM-DEPENDENT RELEASE OF NORADRENALINE FROM PC12 CELLS BY BOTULINUM TYPE-A NEUROTOXIN - LONG-TERM EFFECTS OF THE NEUROTOXIN ON INTACT-CELLS [J].