ULTRAVIOLET INACTIVATION OF AVIAN-SARCOMA VIRUSES - BIOLOGICAL AND BIOCHEMICAL-ANALYSIS

被引:12
作者
OWADA, M
IHARA, S
TOYOSHIMA, K
KOZAI, Y
SUGINO, Y
机构
[1] OSAKA UNIV, RES INST MICROBIAL DIS, DEPT TUMOR VIRUSES, SUITA, OSAKA, JAPAN
[2] TAKEDA CHEM IND LTD, RES & DEV DIV, BIOL RES LABS, HIGASHIYODOGAWA, OSAKA, JAPAN
关键词
D O I
10.1016/0042-6822(76)90499-2
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The rate of inactivation by UV light of the focus-forming capacity of avian sarcoma virus was almost the same as that of the virus-producing capacity, measured as plaque formation. No significant difference was observed in inactivation of the transforming capacity assayed on C/BE chick embryo fibroblasts (CEF), which carry endogenous avian tumor virus DNA, and on duck embryo fibroblasts (DEF), which are devoid of this DNA. All foci induced by nonirradiated virus produced infectious sarcoma virus, but some of the foci induced by UV-irradiated virus did not produce infectious virus of either transforming or transformation-defective type. The proportion of nonproducer foci was 3.4 times more in DEF than in gs- chf- CEF. RNAs extracted from UV-irradiated virions by sodium dodecyl sulfate (SDS) treatment were found to be composed of 60-70 S and 4 S RNAs by analysis in a sucrose gradient containing 0.5% SDS. The large RNA became hydrophobic after irradiation and was sedimented with SDS by addition of 1 drop of saturated potassium chloride solution. This RNA was not dissociated into 30-40 S components by heating at 100.degree. C for 45 s, unlike 60-70 S RNA from unirradiated virions. After SDS-Pronase treatment, the 60-70 S RNA from UV-irradiated virions no longer had these altered characteristics. Reverse transcriptase activity with the endogenous template decreased in parallel with increase in the UV dose. The reduction rate was similar to that assayed with exogenous template or in the presence of actinomycin D. RNA damage may not be the only cause of virus inactivation by UV light.
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页码:710 / 718
页数:9
相关论文
共 33 条
[11]   GENETIC RECOMBINATION WITH AVIAN TUMOR-VIRUS [J].
KAWAI, S ;
HANAFUSA, H .
VIROLOGY, 1972, 49 (01) :37-&
[12]   PLAQUE ASSAY FOR SOME STRAINS OF AVIAN LEUKOSIS VIRUS [J].
KAWAI, S ;
HANAFUSA, H .
VIROLOGY, 1972, 48 (01) :126-&
[13]   RADIATION STUDIES OF AVIAN TUMOR VIRUSES AND OF NEWCASTLE DISEASE VIRUS [J].
LEVINSON, W ;
RUBIN, H .
VIROLOGY, 1966, 28 (04) :533-&
[14]   EFFECT OF UV LIGHT ON RNA-DIRECTED DNA-POLYMERASE ACTIVITY OF MURINE ONCORNAVIRUSES [J].
LOVINGER, GG ;
LING, HP ;
GILDEN, RV ;
HATANAKA, M .
JOURNAL OF VIROLOGY, 1975, 15 (05) :1273-1275
[15]   STRUCTURE AND MOLECULAR-WEIGHT OF 60-70S RNA AND 30-40S RNA OF ROUS-SARCOMA VIRUS [J].
MANGEL, WF ;
DELIUS, H ;
DUESBERG, PH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1974, 71 (11) :4541-4545
[16]   EFFECT OF ULTRAVIOLET-LIGHT ON MENGOVIRUS - FORMATION OF URACIL DIMERS, INSTABILITY AND DEGRADATION OF CAPSID, AND COVALENT LINKAGE OF PROTEIN TO VIRAL-RNA [J].
MILLER, RL ;
PLAGEMAN.PG .
JOURNAL OF VIROLOGY, 1974, 13 (03) :729-739
[17]   ANALYSIS ON REPRODUCING AND CELL-TRANSFORMING CAPACITIES OF A TEMPERATURE SENSITIVE MUTANT (TS 334) OF AVIAN SARCOMA VIRUS-B77 [J].
OWADA, M ;
TOYOSHIM.K .
VIROLOGY, 1973, 54 (01) :170-178
[18]   AVIAN LEUKOSIS VIRUS ASSOCIATED WITH STOCKS OF ROUS SARCOMA VIRUS [J].
RUBIN, H ;
VOGT, PK .
VIROLOGY, 1962, 17 (01) :184-&
[20]   A RE-EXAMINATION OF MOLECULAR WEIGHT OF POLIOVIRUS RNA [J].
TANNOCK, GA ;
GIBBS, AJ ;
COOPER, PD .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1970, 38 (02) :298-&