Adenosine promotes neuronal recovery from reactive oxygen species induced lesion in rat hippocampal slices

被引:45
作者
Almeida, CG [1 ]
de Mendonça, A [1 ]
Cunha, RA [1 ]
Ribeiro, JA [1 ]
机构
[1] Fac Med Lisbon, Neurosci Lab, P-1649028 Lisbon, Portugal
关键词
adenosine; neuroprotection; reactive oxygen species; hippocampus; A(1) receptors; A(2A) receptors; dipropylcyclopentylxanthine;
D O I
10.1016/S0304-3940(02)01478-7
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Reactive oxygen species (ROS) are believed to be involved in the pathogenesis of several neurological disorders. We now tested whether the endogenous neuroprotective substance, adenosine, attenuates the cell damage induced by ROS. In rat hippocampal slices, the xanthine oxidase (40 mU/ml) plus xanthine (I mM) (X/XO) system produced a 27.8 +/- 7.3% (n = 3) increase in ROS, measured by fluorimetry with 2',7'-dichlorodihydrofluorescein, a 246.9 +/- 18.4% (n = 6) increase in the release of tritiated adenosine, and a decrease in synaptic transmission that fully recovered after washout. In the presence of the adenosine A, receptor selective antagonist, 1,3-dipropyl-8-cyclopentylxanthine (100 nM), X/XO induced a similar inhibition, however synaptic transmission only recovered to 70.7 +/- 5.8% of control (n = 5). The blockade of A(2A) receptors was devoid of effect (it = 4). Adenosine is released by ROS-generating systems, and attenuates the deleterious cellular consequences of ROS through A, receptor activation. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:127 / 130
页数:4
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