Sphingosine kinase regulates oxidized low density lipoprotein-mediated calcium oscillations and macrophage survival

被引:5
作者
Chen, Johnny H. [1 ]
Riazy, Maziar [1 ]
Wang, Shih Wei [1 ]
Dai, Jiazhen Minnie [1 ]
Duronio, Vincent [1 ]
Steinbrecher, Urs P. [1 ]
机构
[1] Univ British Columbia, Dept Med, Vancouver, BC, Canada
关键词
sphingosine-1-phosphate; calcium; foam cells; plaque stability; MUSCLE CELL-PROLIFERATION; COLONY-STIMULATING FACTOR; CA2+ TRANSPORT ATPASES; SPHINGOLIPID METABOLISM; SIGNALING PATHWAYS; ENDOTHELIAL-CELLS; GROWTH; SPHINGOSINE-1-PHOSPHATE; RECEPTOR; ACTIVATION;
D O I
10.1194/jlr.M000398
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We recently reported that oxidized LDL (oxLDL) induces an oscillatory increase in intracellular calcium ([Ca2+](i)) levels in macrophages. Furthermore, we have shown that these [Ca2+](i) oscillations mediate oxLDL's ability to inhibit macrophage apoptosis in response to growth factor deprivation. However, the signal transduction pathways by which oxLDL induces [Ca2+](i) oscillations have not been elucidated. In this study, we show that these oscillations are mediated in part by intracellular mechanisms, as depleting extracellular Ca2+ did not completely abolish the effect. Inhibiting sarco-endoplasmic reticulum ATPase (SERCA) completely blocked [Ca2+](i) oscillations, suggesting a role for Ca2+ reuptake by the ER. The addition of oxLDL resulted in an almost immediate activation of sphingosine kinase (SK), which can increase sphingosine-1-phosphate (S1P) levels by phosphorylating sphingosine. Moreover, S1P was shown to be as effective as oxLDL in blocking macrophage apoptosis and producing [Ca2+](i) oscillations. This suggests that the mechanism in which oxLDL generates [Ca2+](i) oscillations may be 1) activation of SK, 2) SK-mediated increase in S1P levels, 3) S1P-mediated Ca2+ release from intracellular stores, and 4) SERCA-mediated Ca2+ reuptake back into the ER.-Chen, J. H., M. Riazy, S. W. Wang, J. M. Dai, V. Duronio, and U. P. Steinbrecher. Sphingosine kinase regulates oxidized low density lipoprotein-mediated calcium oscillations and macrophage survival. J. Lipid Res. 2010. 51: 991-998.
引用
收藏
页码:991 / 998
页数:8
相关论文
共 60 条
[1]   Formyl peptide receptor signaling in HL-60 cells through sphingosine kinase [J].
Alemany, R ;
Heringdorf, DMZ ;
van Koppen, CJ ;
Jakobs, KH .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (07) :3994-3999
[2]   Autocrine and paracrine roles of sphingosine-1-phosphate [J].
Alvarez, Sergio E. ;
Milstien, Sheldon ;
Spiegel, Sarah .
TRENDS IN ENDOCRINOLOGY AND METABOLISM, 2007, 18 (08) :300-307
[3]   Role of sphingosine 1-phosphate in the mitogenesis induced by oxidized low density lipoprotein in smooth muscle cells via activation of sphingomyelinase, ceramidase, and sphingosine kinase [J].
Augé, N ;
Nikolova-Karakashian, M ;
Carpentier, S ;
Parthasarathy, S ;
Négre-Salvayre, A ;
Salvayre, R ;
Merrill, AH ;
Levade, T .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (31) :21533-21538
[4]   Oxidized LDL-induced smooth muscle cell proliferation involves the EGF receptor/PI-3 kinase/Akt and the sphingolipid signaling pathways [J].
Auge, N ;
Garcia, V ;
Maupas-Schwalm, F ;
Levade, T ;
Salvayre, R ;
Negre-Salvayre, A .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2002, 22 (12) :1990-1995
[5]   Calcium signalling: Dynamics, homeostasis and remodelling [J].
Berridge, MJ ;
Bootman, MD ;
Roderick, HL .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2003, 4 (07) :517-529
[6]   The versatility and universality of calcium signalling [J].
Berridge, MJ ;
Lipp, P ;
Bootman, MD .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2000, 1 (01) :11-21
[7]   Calcium - a life and death signal [J].
Berridge, MJ ;
Bootman, MD ;
Lipp, P .
NATURE, 1998, 395 (6703) :645-648
[8]   Induction of murine macrophage growth by oxidized low density lipoprotein is mediated by granulocyte macrophage colony-stimulating factor [J].
Biwa, T ;
Hakamata, H ;
Sakai, M ;
Miyazaki, A ;
Suzuki, H ;
Kodama, T ;
Shichiri, M ;
Horiuchi, S .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (43) :28305-28313
[9]   Expression and functional characterization of SCaMPER: a sphingolipid-modulated calcium channel of cardiomyocytes [J].
Cavalli, AL ;
O'Brien, NW ;
Barlow, SB ;
Betto, R ;
Glembotski, CC ;
Palade, PT ;
Sabbadini, RA .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2003, 284 (03) :C780-C790
[10]   Oxidized LDL-Mediated Macrophage Survival Involves Elongation Factor-2 Kinase [J].
Chen, Johnny H. ;
Riazy, Maziar ;
Smith, Ewan M. ;
Proud, Christopher G. ;
Steinbrecher, Urs P. ;
Duronio, Vincent .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2009, 29 (01) :92-U238