The use of confocal microscopy in the investigation of cell structure and function in the heart, vascular endothelium and smooth muscle cells

被引：105

作者：

Bkaily, G ^{[1
]}

Pothier, P ^{[1
]}

DOrleansJuste, P ^{[1
]}

Simaan, M ^{[1
]}

Jacques, D ^{[1
]}

Jaalouk, D ^{[1
]}

Belzile, F ^{[1
]}

Hassan, G ^{[1
]}

Boutin, C ^{[1
]}

Haddad, G ^{[1
]}

Neugebauer, W ^{[1
]}

机构：

[1] UNIV SHERBROOKE,FAC MED,DEPT PHARMACOL,MRCC GROUP IMMUNOCARDIOVASC INTERACT,SHERBROOKE,PQ J1H 5N4,CANADA

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 1997年 / 172卷 / 1-2期

关键词：

heart; vascular endothelium; vascular smooth muscle; confocal microscopy; pH; calcium; sodium; voltage probe; endothelin-1; Angiotensin II; PAF; nucleus; mitochondria; SR; cardiomyopathy; cells interaction; R-type Ca2+ channel; excitation-contraction coupling; dystrophic mouse;

D O I：

10.1023/A:1006840228104

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In recent years, fluorescence microscopy imaging has become an important tool for studying cell structure and function. This non invasive technique permits characterization, localisation and qualitative quantification of free ions, messengers, pH, voltage and a pleiad of other molecules constituting living cells. In this paper, we present results using various commercially available fluorescent probes as well as some developed in our laboratory and discuss the advantages and limitations of these probes in confocal microscopy studies of the cardiovascular system.

引用

页码：171 / 194

页数：24

共 48 条

[21] APPLICATIONS OF RATIO FLUORESCENCE MICROSCOPY IN THE STUDY OF CELL PHYSIOLOGY [J].