The use of confocal microscopy in the investigation of cell structure and function in the heart, vascular endothelium and smooth muscle cells

被引：105

作者：

Bkaily, G ^{[1
]}

Pothier, P ^{[1
]}

DOrleansJuste, P ^{[1
]}

Simaan, M ^{[1
]}

Jacques, D ^{[1
]}

Jaalouk, D ^{[1
]}

Belzile, F ^{[1
]}

Hassan, G ^{[1
]}

Boutin, C ^{[1
]}

Haddad, G ^{[1
]}

Neugebauer, W ^{[1
]}

机构：

[1] UNIV SHERBROOKE,FAC MED,DEPT PHARMACOL,MRCC GROUP IMMUNOCARDIOVASC INTERACT,SHERBROOKE,PQ J1H 5N4,CANADA

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 1997年 / 172卷 / 1-2期

关键词：

heart; vascular endothelium; vascular smooth muscle; confocal microscopy; pH; calcium; sodium; voltage probe; endothelin-1; Angiotensin II; PAF; nucleus; mitochondria; SR; cardiomyopathy; cells interaction; R-type Ca2+ channel; excitation-contraction coupling; dystrophic mouse;

D O I：

10.1023/A:1006840228104

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In recent years, fluorescence microscopy imaging has become an important tool for studying cell structure and function. This non invasive technique permits characterization, localisation and qualitative quantification of free ions, messengers, pH, voltage and a pleiad of other molecules constituting living cells. In this paper, we present results using various commercially available fluorescent probes as well as some developed in our laboratory and discuss the advantages and limitations of these probes in confocal microscopy studies of the cardiovascular system.

引用

页码：171 / 194

页数：24

共 48 条

[1] THE NUCLEUS IS INSULATED FROM LARGE CYTOSOLIC CALCIUM-ION CHANGES [J].

ALMOHANNA, FA ;

CADDY, KWT ;

BOLSOVER, SR .

NATURE, 1994, 367 (6465) :745-750

[2] LASER CONFOCAL SCANNING MICROSCOPY OF THE SURFACE-MEMBRANE T-TUBULAR SYSTEM AND THE SARCOPLASMIC-RETICULUM IN INSECT STRIATED-MUSCLE STAINED WITH DILC18(3) [J].

BAUMANN, O ;

KITAZAWA, T ;

SOMLYO, AP .

JOURNAL OF STRUCTURAL BIOLOGY, 1990, 105 (1-3) :154-161

[3]

BENCHEKROUN MT, 1995, J CARDIOVASC PHARM, V26, P300

[4] PAF ACTIVATION OF A VOLTAGE-GATED R-TYPE CA(2+) CHANNEL IN HUMAN AND CANINE AORTIC ENDOTHELIAL-CELLS [J].

BKAILY, G ;

DORLEANSJUSTE, P ;

NAIK, R ;

PERODIN, J ;

STANKOVA, J ;

ABDULNOUR, E ;

ROLAPLESZCZYNSKI, M .

BRITISH JOURNAL OF PHARMACOLOGY, 1993, 110 (02) :519-520

[5] Implication of the nucleus in excitation contraction coupling of heart cells [J].

Bkaily, G ;

GrosLouis, N ;

Naik, R ;

Jaalouk, D ;

Pothier, P .

MOLECULAR AND CELLULAR BIOCHEMISTRY, 1996, 154 (02) :113-121

[6] ATRIAL-NATRIURETIC-FACTOR BLOCKS THE HIGH-THRESHOLD CA2+ CURRENT AND INCREASES K+ CURRENT IN FETAL SINGLE VENTRICULAR CELLS [J].

BKAILY, G ;

PERRON, N ;

WANG, S ;

SCULPTOREANU, A ;

JACQUES, D ;

MENARD, D .

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 1993, 25 (11) :1305-1316

[7] APAMIN, A HIGHLY POTENT FETAL L-TYPE CA2+ CURRENT BLOCKER IN SINGLE HEART-CELLS [J].

BKAILY, G ;

SCULPTOREANU, A ;

JACQUES, D ;

ECONOMOS, D ;

MENARD, D .

AMERICAN JOURNAL OF PHYSIOLOGY, 1992, 262 (02) :H463-H471

[8]

BKAILY G, 1992, MOL CELL BIOCHEM, V117, P93

[9]

BKAILY G, 1988, MOL CELL BIOCHEM, V80, P59

[10] BETHANIDINE INCREASED NA+ AND CA-2+ CURRENTS AND CAUSED A POSITIVE INOTROPIC EFFECT IN HEART-CELLS [J].

BKAILY, G ;

PAYET, MD ;

BENABDERRAZIK, M ;

RENAUD, JF ;

SAUVE, R ;

BACANER, MB ;

SPERELAKIS, N .

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 1988, 66 (03) :190-196

← 1 2 3 4 5 →