Electron tomography: towards visualizing the molecular organization of the cytoplasm

被引：169

作者：

Baumeister, W ^{[1
]}

机构：

[1] Max Planck Inst Biochem, Dept Biol Struct, D-82152 Martinsried, Germany

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2002年 / 12卷 / 05期

关键词：

D O I：

10.1016/S0959-440X(02)00378-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Electron tomography of thin sections of plastic-em bedded biological material provides valuable insights into the organellar organization of cells, With the advent of automated data-acquisition procedures, it has become possible to study radiation-sensitive specimens, such as frozen-hydrated organelles and whole cells. This opens up exciting perspectives for visualizing the molecular organization of the cytoplasm.

引用

页码：679 / 684

页数：6

共 41 条

[11] RECONSTRUCTION OF 3 DIMENSIONAL STRUCTURES FROM ELECTRON MICROGRAPHS [J].

DEROSIER, DJ ;

KLUG, A .

NATURE, 1968, 217 (5124) :130-&

[12] Noise reduction in electron tomographic reconstructions using nonlinear anisotropic diffusion [J].

Frangakis, AS ;

Hegerl, R .

JOURNAL OF STRUCTURAL BIOLOGY, 2001, 135 (03) :239-250

[13] Electron tomography of ice-embedded prokaryotic cells [J].

Grimm, R ;

Singh, H ;

Rachel, R ;

Typke, D ;

Zillig, W ;

Baumeister, W .

BIOPHYSICAL JOURNAL, 1998, 74 (02) :1031-1042

[14] Improving image quality by zero-loss energy filtering: quantitative assessment by means of image cross-correlation [J].

Grimm, R ;

Typke, D ;

Baumeister, W .

JOURNAL OF MICROSCOPY-OXFORD, 1998, 190 :339-349

[15] ELECTRON MICROSCOPY OF UNSTAINED BIOLOGICAL MATERIAL - POLYTROPIC MONTAGE [J].

HART, RG .

SCIENCE, 1968, 159 (3822) :1464-&

[16] Corneal collagen fibril structure in three dimensions: Structural insights into fibril assembly, mechanical properties, and tissue organization [J].

Holmes, DF ;

Gilpin, CJ ;

Baldock, C ;

Ziese, U ;

Koster, AJ ;

Kadler, KE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (13) :7307-7312

[17]

HOPPE W, 1974, H-S Z PHYSIOL CHEM, V355, P1483

[18] Perspectives of molecular and cellular electron tomography [J].

Koster, AJ ;

Grimm, R ;

Typke, D ;

Hegerl, R ;

Stoschek, A ;

Walz, J ;

Baumeister, W .

JOURNAL OF STRUCTURAL BIOLOGY, 1997, 120 (03) :276-308

[19]

Luther P. K., 1992, ELECTRON TOMOGRAPHY, P39

[20] Topology of the mitochondrial inner membrane: Dynamics and bioenergetic implications [J].

Mannella, CA ;

Pfeiffer, DR ;

Bradshaw, PC ;

Moraru, II ;

Slepchenko, B ;

Loew, LM ;

Hsieh, C ;

Buttle, K ;

Marko, M .

IUBMB LIFE, 2001, 52 (3-5) :93-100

← 1 2 3 4 5 →