Hydroxylation of sesquiterpenes by enzymes from chicory (Cichorium intybus L.) roots.

被引：36

作者：

de Kraker, JW

Schurink, M

Franssen, MCR

König, WA

de Groot, A

Bouwmeester, HJ

机构：

[1] Univ Wageningen & Res Ctr, Organ Chem Lab, NL-6703 HB Wageningen, Netherlands

[2] Plant Res Int, NL-6700 AA Wageningen, Netherlands

[3] Univ Hamburg, Inst Organ Chem, D-20146 Hamburg, Germany

来源：

TETRAHEDRON | 2003年 / 59卷 / 03期

关键词：

plant; enzyme; hydroxylation; sesquiterpene; bioconversion; nootkatone; cytochrome P450; dehydrogenase;

D O I：

10.1016/S0040-4020(02)01479-5

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A microsomal enzyme preparation of chicory roots catalyses the hydroxylation of various sesquiterpene olefins in the presence of NADPH. Most of these hydroxylations take place at an isopropenyl or isopropylidene group. The number of products obtained from any of the substrates is confined to one or, in a few cases, two sesquiterpene alcohols. In addition, the conversion of (+)-valencene into nootkatone through beta-nootkatol was observed. The involvement of (+)-germacrene A hydroxylase (a cytochrome P450 enzyme) and other enzymes of sesquiterpene lactone biosynthesis in these reactions is discussed. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：409 / 418

页数：10

共 30 条

[21] BIOCONVERSION OF SESQUITERPENES [J].