'Semifree-floating' treatment: A simple and fast method to process consecutive sections for immunohistochemistry and neuronal tracing

被引：61

作者：

Herzog, A ^{[1
]}

Brosamle, C ^{[1
]}

机构：

[1] UNIV ZURICH,BRAIN RES INST,CH-8029 ZURICH,SWITZERLAND

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1997年 / 72卷 / 01期

关键词：

neuroanatomy; immunohistochemistry; neuronal tracing; 3D reconstruction; thick sections; confocal microscopy;

D O I：

10.1016/S0165-0270(96)00156-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described which allows for histochemical processing of thick (50-200 mu m) and consecutive sections of neural tissue, a prerequisite for many neuroanatomical studies. Two examples are given: (A) biotin-dextran-amine (BDA) tracing of neuronal connections in 50-100 mu m thick vibratome sections of the adult rat brain and (B) immunohistochemical analysis of tyrosine hydroxylase-positive bulbospinal fibers in 50 mu m thick cryosections of spinal cord. (C) 1997 Elsevier Science B.V.

引用

页码：57 / 63

页数：7

共 11 条

[1] EFFECT OF DOPA ON SPINAL CORD .3. DEPOLARIZATION EVOKED IN CENTRAL TERMINALS OF IPSILATERAL IA AFFERENTS BY VOLLEYS IN FLEXOR REFLEX AFFERENTS [J].