BIOTIN DEXTRAN - A SENSITIVE ANTEROGRADE TRACER FOR NEUROANATOMICAL STUDIES IN RAT AND MONKEY

被引：200

作者：

BRANDT, HM ^{[1
]}

APKARIAN, AV ^{[1
]}

机构：

[1] SUNY HLTH SCI CTR,DEPT NEUROSURG,766 IRVING AVE,SYRACUSE,NY 13210

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1992年 / 45卷 / 1-2期

关键词：

BIOTIN; DEXTRAN; RETROGRADE LABEL; AXONAL LABEL; DIAMINOBENZIDINE; SPINAL CORD;

D O I：

10.1016/0165-0270(92)90041-B

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The properties of a new anterograde tracer, biotin-dextran (BD), are reported. Iontophoretic or pressure injections of BD in the spinal cord of rats and monkeys revealed terminal-like BD label in many regions of the brain. The BD label was visualized by an avidin-biotin reaction combined with diaminobenzidine, with and without nickel enhancement. This reaction resulted in permanent label and revealed the fine morphology of terminal boutons and en-passant endings. Biotin-dextran is an excellent anterograde tracer that can also be visualized at the electron microscopic level, used in combination with other retrograde and anterograde tracers and with immunohistochemical labeling of neurotransmitters.

引用

页码：35 / 40

页数：6

共 20 条

[1] PRIMATE SPINOTHALAMIC PATHWAYS .3. THALAMIC TERMINATIONS OF THE DORSOLATERAL AND VENTRAL SPINOTHALAMIC PATHWAYS [J].

APKARIAN, AV ;

HODGE, CJ .

JOURNAL OF COMPARATIVE NEUROLOGY, 1989, 288 (03) :493-511

[2]

APKARIAN AV, 1991, IBRO ABST, V3, P54

[3]

CLIFFER KD, 1991, J NEUROSCI, V11, P852

[4]

Emmers R., 1963, STEREOTAXIC ATLAS BR

[5] AN ANTEROGRADE NEUROANATOMICAL TRACING METHOD THAT SHOWS THE DETAILED MORPHOLOGY OF NEURONS, THEIR AXONS AND TERMINALS - IMMUNOHISTOCHEMICAL LOCALIZATION OF AN AXONALLY TRANSPORTED PLANT LECTIN, PHASEOLUS-VULGARIS LEUKOAGGLUTININ (PHA-L) [J].

GERFEN, CR ;

SAWCHENKO, PE .

BRAIN RESEARCH, 1984, 290 (02) :219-238

[6]

HAZRATI LN, 1992, BRAIN RES, V596, P336

[7] A NOTE ON THE USE OF BIOCYTIN IN ANTEROGRADE TRACING STUDIES IN THE CENTRAL-NERVOUS-SYSTEM - APPLICATION AT BOTH LIGHT AND ELECTRON-MICROSCOPIC LEVEL [J].

IZZO, PN .

JOURNAL OF NEUROSCIENCE METHODS, 1991, 36 (2-3) :155-166

[8] BIOCYTIN - A VERSATILE ANTEROGRADE NEUROANATOMICAL TRACT-TRACING ALTERNATIVE [J].

KING, MA ;

LOUIS, PM ;

HUNTER, BE ;

WALKER, DW .

BRAIN RESEARCH, 1989, 497 (02) :361-367

[9] MORPHOLOGICAL DETAILS OF PRIMATE AXONS AND DENDRITES REVEALED BY EXTRACELLULAR INJECTION OF BIOCYTIN - AN ECONOMIC AND RELIABLE ALTERNATIVE TO PHA-L [J].

LACHICA, EA ;

MAVITYHUDSON, JA ;

CASAGRANDE, VA .

BRAIN RESEARCH, 1991, 564 (01) :1-11

[10] AFFERENTS TO THE CEREBELLAR NUCLEI FROM THE CERVICAL ENLARGEMENT IN THE RAT, AS DEMONSTRATED WITH THE PHASEOLUS-VULGARIS LEUKOAGGLUTININ METHOD [J].

MATSUSHITA, M ;

YAGINUMA, H .

NEUROSCIENCE LETTERS, 1990, 113 (03) :253-259

← 1 2 →