Immunohistochemical analysis of cyclooxygenase-2 expression in pancreatic tumors

被引：71

作者：

Koshiba, T ^{[1
]}

Hosotani, R ^{[1
]}

Miyamoto, Y ^{[1
]}

Wada, M ^{[1
]}

Lee, JU ^{[1
]}

Fujimoto, K ^{[1
]}

Tsuji, S ^{[1
]}

Nakajima, S ^{[1
]}

Doi, R ^{[1
]}

Imamura, M ^{[1
]}

机构：

[1] Kyoto Univ, Dept Surg & Surg Basic Sci, Sakyo Ku, Kyoto 6064897, Japan

来源：

INTERNATIONAL JOURNAL OF PANCREATOLOGY | 1999年 / 26卷 / 02期

关键词：

immunohistochemistry; immunoblot analysis; Cox-2; pancreatic cancer; intraductal papillary-mucinous tumor;

D O I：

10.1007/BF02781733

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background. A considerable amount of evidence collected from several experimental systems and clinical studies with nonsteroidal Anti-inflammatory drugs (NSAIDs) indicates that Cox-2 may play a major role in colorectal tumorigenesis, but little information about Cox-2 expression in pancreatic tumors is available. In this study, we investigated Cox-2 expression by means of both immunohistochemical analysis and immunoblot analysis in pancreatic tumors. Methods. Fifty invasive ductal adenocarcinomas and 26 intraductal papillary-mucinous tumors (IPMTs) were used for immunohistochemical analysis, and five pancreatic cancer tissues and five pancreatic cancer cell lines for immunoblot analysis. Results. Cox-2 was expressed in 72% of the invasive ductal adenocarcinomas, 31% of intraductal papillary-mucinous adenocarcinomas, and none of intraductal papillary-mucinous adenomas. The expression rate of Cox-2 in intraductal papillary-mucinous adenocarcinomas was significantly higher than that in intraductal papillary-mucinous adenomas, and that in invasive ductal adenocarcinomas was significantly higher than that in intraductal papillary-mucinous carcinomas. However, there was no significant correlation between Cox-2 expression and the prognosis and clinicopathological factors. Immunoblot analysis identified Cox-2 in all of pancreatic cancer tissues and 60% of cell lines. Conclusion. The biological role of cyclooxygenase-2 (Cox-2) in carcinoma cells should be investigated with reference to the cancer progression of the pancreas.

引用

页码：69 / 76

页数：8