3D organization of synthetic and scrambled chromosomes

被引：124

作者：

Mercy, Guillaume ^{[1
,2
,3
]}

Mozziconacci, Julien ^{[4
]}

Scolari, Vittore F. ^{[1
,2
]}

Yang, Kun ^{[5
,6
]}

Zhao, Guanghou ^{[7
]}

Thierry, Agnes ^{[1
,2
]}

Luo, Yisha ^{[8
]}

Mitchell, Leslie A. ^{[9
,10
]}

Shen, Michael ^{[9
,10
]}

Shen, Yue ^{[8
,11
,12
]}

Walker, Roy ^{[8
]}

Zhang, Weimin ^{[7
]}

Wu, Yi ^{[13
]}

Xie, Ze-Xiong ^{[13
]}

Luo, Zhouqing ^{[7
]}

Cai, Yizhi ^{[8
]}

Dai, Junbiao ^{[7
]}

Yang, Huanming ^{[11
,14
]}

Yuan, Ying-Jin ^{[13
]}

Boeke, Jef D. ^{[9
,10
]}

Bader, Joel S. ^{[5
,6
]}

Muller, Heloise ^{[1
,2
,15
]}

Koszul, Romain ^{[1
,2
]}

机构：

[1] Inst Pasteur, Dept Genomes & Genet, Spatial Regulat Genomes, F-75015 Paris, France

[2] CNRS, UMR3525, F-75015 Paris, France

[3] Univ Paris 06, Univ Pierre & Marie Curie, Sorbonne Univ, F-75005 Paris, France

[4] Sorbonne Univ, Univ Paris 6, Univ Pierre & Marie Curie, Lab Phys Theor Matiere Condensee,CNRS,UMR7600, Paris, France

[5] Johns Hopkins Univ, Whiting Sch Engn, Dept Biomed Engn, Baltimore, MD 21218 USA

[6] Johns Hopkins Univ, Whiting Sch Engn, High Throughput Biol Ctr, Baltimore, MD 21218 USA

[7] Tsinghua Univ, Key Lab Ind Biocatalysis, Minist Educ, Key Lab Bioinformat,Ctr Synthet & Syst Biol,Sch L, Beijing 100084, Peoples R China

[8] Univ Edinburgh, Sch Biol Sci, Edinburgh EH9 3BF, Midlothian, Scotland

[9] NYU, Inst Syst Genet, Langone Med Ctr, 550 1St Ave, New York, NY 10016 USA

[10] NYU, Dept Biochem & Mol Pharmacol, Langone Med Ctr, 550 1St Ave, New York, NY 10016 USA

[11] BGI Shenzhen, Shenzhen 518083, Peoples R China

[12] BGI Qingdao, Qingdao 266555, Peoples R China

[13] Tianjin Univ, Key Lab Syst Bioengn, Minist Educ,Sch Chem Engn & Technol, SynBio Res Platform,Collaborat Innovat Ctr Chem S, Tianjin 300072, Peoples R China

[14] James D Watson Inst Genome Sci, Hangzhou 310058, Zhejiang, Peoples R China

[15] Inst Curie, Dynam Noyau UMR3664, Paris, France

来源：

SCIENCE | 2017年 / 355卷 / 6329期

基金：

英国生物技术与生命科学研究理事会; 美国国家科学基金会; 欧洲研究理事会;

关键词：

IN-VIVO; YEAST; PRINCIPLES;

D O I：

10.1126/science.aaf4597

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Although the design of the synthetic yeast genome Sc2.0 is highly conservative with respect to gene content, the deletion of several classes of repeated sequences and the introduction of thousands of designer changes may affect genome organization and potentially alter cellular functions. We report here the Hi-C-determined three-dimensional (3D) conformations of Sc2.0 chromosomes. The absence of repeats leads to a smoother contact pattern and more precisely tractable chromosome conformations, and the largescale genomic organization is globally unaffected by the presence of synthetic chromosome(s). Two exceptions are synIII, which lacks the silent mating-type cassettes, and synXII, specifically when the ribosomal DNA is moved to another chromosome. We also exploit the contact maps to detect rearrangements induced in SCRaMbLE (synthetic chromosome rearrangement and modification by loxP-mediated evolution) strains.

引用

页数：8

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