Chromosome looping in yeast: telomere pairing and coordinated movement reflect anchoring efficiency and territorial organization

被引:159
作者
Bystricky, K
Laroche, T
van Houwe, G
Blaszczyk, M
Gasser, SM [1 ]
机构
[1] Univ Geneva, Dept Mol Biol, CH-1211 Geneva 4, Switzerland
[2] Univ Geneva, NCCR Frontiers Genet, CH-1211 Geneva 4, Switzerland
[3] Univ Lausanne, Inst Appl Math, CH-1015 Lausanne, Switzerland
关键词
D O I
10.1083/jcb.200409091
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Long-range chromosome organization is known to influence nuclear function. Budding yeast centromeres cluster near the spindle pole body, whereas telomeres are grouped in five to eight perinuclear foci. Using live microscopy, we examine the relative positions of right and left telomeres of several yeast chromosomes. Integrated lac and tet operator arrays are visualized by their respective repressor fused to CFP and YFP in interphase yeast cells. The two ends of chromosomes 3 and 6 interact significantly but transiently, forming whole chromosome loops. For chromosomes 5 and 14, end-to-end interaction is less frequent, yet telomeres are closer to each other than to the centromere, suggesting that yeast chromosomes fold in a Rabl-like conformation. Disruption of telomere anchoring by deletions of YKU70 or SIR4 significantly compromises contact between two linked telomeres. These mutations do not, however, eliminate coordinated movement of telomere (Tel) 6R and Tel6L, which we propose stems from the territorial organization of yeast chromosomes.
引用
收藏
页码:375 / 387
页数:13
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