Long-range chromosome organization is known to influence nuclear function. Budding yeast centromeres cluster near the spindle pole body, whereas telomeres are grouped in five to eight perinuclear foci. Using live microscopy, we examine the relative positions of right and left telomeres of several yeast chromosomes. Integrated lac and tet operator arrays are visualized by their respective repressor fused to CFP and YFP in interphase yeast cells. The two ends of chromosomes 3 and 6 interact significantly but transiently, forming whole chromosome loops. For chromosomes 5 and 14, end-to-end interaction is less frequent, yet telomeres are closer to each other than to the centromere, suggesting that yeast chromosomes fold in a Rabl-like conformation. Disruption of telomere anchoring by deletions of YKU70 or SIR4 significantly compromises contact between two linked telomeres. These mutations do not, however, eliminate coordinated movement of telomere (Tel) 6R and Tel6L, which we propose stems from the territorial organization of yeast chromosomes.
机构:
Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, ScotlandWestern Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland
Croft, JA
;
Bridger, JM
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Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, ScotlandWestern Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland
Bridger, JM
;
Boyle, S
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Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, ScotlandWestern Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland
Boyle, S
;
Perry, P
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Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, ScotlandWestern Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland
Perry, P
;
Teague, P
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Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, ScotlandWestern Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland
Teague, P
;
Bickmore, WA
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Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, ScotlandWestern Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland
机构:
Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, ScotlandWestern Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland
Croft, JA
;
Bridger, JM
论文数: 0引用数: 0
h-index: 0
机构:
Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, ScotlandWestern Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland
Bridger, JM
;
Boyle, S
论文数: 0引用数: 0
h-index: 0
机构:
Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, ScotlandWestern Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland
Boyle, S
;
Perry, P
论文数: 0引用数: 0
h-index: 0
机构:
Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, ScotlandWestern Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland
Perry, P
;
Teague, P
论文数: 0引用数: 0
h-index: 0
机构:
Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, ScotlandWestern Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland
Teague, P
;
Bickmore, WA
论文数: 0引用数: 0
h-index: 0
机构:
Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, ScotlandWestern Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland