DEAR1, a transcriptional repressor of DREB protein that mediates plant defense and freezing stress responses in Arabidopsis

被引：131

作者：

Tsutsui, Tomokazu ^{[1
]}

Kato, Wataru ^{[1
]}

Asada, Yutaka ^{[1
]}

Sako, Kaori ^{[1
]}

Sato, Takeo ^{[1
]}

Sonoda, Yutaka ^{[1
]}

Kidokoro, Satoshi ^{[2
]}

Yamaguchi-Shinozaki, Kazuko ^{[2
,3
]}

Tamaoki, Masanori ^{[4
]}

Arakawa, Keita ^{[5
]}

Ichikawa, Takanari ^{[6
]}

Nakazawa, Miki ^{[6
]}

Seki, Motoaki ^{[7
]}

Shinozaki, Kazuo ^{[6
]}

Matsui, Minami ^{[6
]}

Ikeda, Akira ^{[1
]}

Yamaguchi, Junji ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Life Sci, Fac Adv Life Sci, Kita Ku, Sapporo, Hokkaido 0600810, Japan

[2] Univ Tokyo, Grad Sch Agr & Life Sci, Lab Plant Mol Physiol, Bunkyo Ku, Tokyo 1138657, Japan

[3] Japan Int Res Ctr Agr Sci, Biol Resources Div, Tsukuba, Ibaraki 3058686, Japan

[4] Natl Inst Environm Studies, Biodivers Conservat Res Project, Tsukuba, Ibaraki 3058506, Japan

[5] Hokkaido Univ, Grad Sch Agr, Kita Ku, Sapporo, Hokkaido 0608589, Japan

[6] RIKEN, Yokohama Inst, PSC, Plant Funct Genom Res Grp, Kanagawa 2300045, Japan

[7] RIKEN, Yokohama Inst, PSC, Plant Genom Network Res Grp, Kanagawa 2300045, Japan

来源：

JOURNAL OF PLANT RESEARCH | 2009年 / 122卷 / 06期

关键词：

Arabidopsis; DREB1/CBF family; Biotic stress; Abiotic stress; Salicylic acid; EAR motif; SYSTEMIC ACQUIRED-RESISTANCE; PROGRAMMED CELL-DEATH; SALICYLIC-ACID; COLD-ACCLIMATION; LOW-TEMPERATURE; HYPERSENSITIVE RESPONSE; SIGNALING PATHWAYS; GENE-EXPRESSION; EAR-MOTIF; TOLERANCE;

D O I：

10.1007/s10265-009-0252-6

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Plants have evolved intricate mechanisms to respond and adapt to a wide variety of biotic and abiotic stresses in their environment. The Arabidopsis DEAR1 (DREB and EAR motif protein 1; At3g50260) gene encodes a protein containing significant homology to the DREB1/CBF (dehydration-responsive element binding protein 1/C-repeat binding factor) domain and the EAR (ethylene response factor-associated amphiphilic repression) motif. We show here that DEAR1 mRNA accumulates in response to both pathogen infection and cold treatment. Transgenic Arabidopsis overexpressing DEAR1 (DEAR1ox) showed a dwarf phenotype and lesion-like cell death, together with constitutive expression of PR genes and accumulation of salicylic acid. DEAR1ox also showed more limited P. syringae pathogen growth compared to wild-type, consistent with an activated defense phenotype. In addition, transient expression experiments revealed that the DEAR1 protein represses DRE/CRT (dehydration-responsive element/C-repeat)-dependent transcription, which is regulated by low temperature. Furthermore, the induction of DREB1/CBF family genes by cold treatment was suppressed in DEAR1ox, leading to a reduction in freezing tolerance. These results suggest that DEAR1 has an upstream regulatory role in mediating crosstalk between signaling pathways for biotic and abiotic stress responses.

引用

页码：633 / 643

页数：11