In vivo trafficking and targeted delivery of magnetically labeled stem cells

被引：150

作者：

Arbab, AS ^{[1
]}

Jordan, EK ^{[1
]}

Wilson, LB ^{[1
]}

Yocum, GT ^{[1
]}

Lewis, BK ^{[1
]}

Frank, JA ^{[1
]}

机构：

[1] NIH, Expt Neuroimaging Sect, Lab Diagnost Radiol Res, Bethesda, MD 20892 USA

来源：

HUMAN GENE THERAPY | 2004年 / 15卷 / 04期

关键词：

D O I：

10.1089/104303404322959506

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Targeted delivery of intravenously administered genetically altered cells or stem cells is still in an early stage of investigation. We developed a method of delivering iron oxide (ferumoxide)-labeled mesenchymal stem cells (MSCs) to a targeted area in an animal model by applying an external magnet. Rats with or without an external magnet placed over the liver were injected intravenously with ferumoxide-labeled MSCs and magnetic resonance imaging (MRI) signal intensity (SI) changes, iron concentration, and concentration of MSCs in the liver were monitored at different time points. SI decreased in the liver after injection of MSCs and returned gradually to that of control rat livers at approximately day 29. SI decreases were greater in rats with external magnets. Higher iron concentration and increased labeled cell numbers were detected in rat livers with external magnets. The external magnets influenced the movement of labeled MSCs such that the cells were retained in the region of interest. These results potentially open a new area of investigation for delivering stem cells or genetically altered cells.

引用

页码：351 / 360

页数：10

共 37 条

[21]

Kircher MF, 2003, CANCER RES, V63, P6838

[22] Adult stem cells and tissue repair [J].

Körbling, M ;

Estrov, Z ;

Champlin, R .

BONE MARROW TRANSPLANTATION, 2003, 32 :S23-S24

[23] In vivo magnetic resonance imaging of mesenchymal stem cells in myocardial infarction [J].

Kraitchman, DL ;

Heldman, AW ;

Atalar, E ;

Amado, LC ;

Martin, BJ ;

Pittenger, MF ;

Hare, JM ;

Bulte, JWM .

CIRCULATION, 2003, 107 (18) :2290-2293

[24]

MartinHenao GA, 1996, BONE MARROW TRANSPL, V18, P603

[25] MRI of insulitis in autoimmune diabetes [J].

Moore, A ;

Sun, PZ ;

Cory, D ;

Högemann, D ;

Weissleder, R ;

Lipes, MA .

MAGNETIC RESONANCE IN MEDICINE, 2002, 47 (04) :751-758

[26] Hematopoietic stem cell transplantation for multiple sclerosis: current status and future challenges [J].

Muraro, PA ;

Ingoni, RC ;

Martin, R .

CURRENT OPINION IN NEUROLOGY, 2003, 16 (03) :299-305

[27] Bone marrow cells regenerate infarcted myocardium [J].

Orlic, D ;

Kajstura, J ;

Chimenti, S ;

Jakoniuk, I ;

Anderson, SM ;

Li, BS ;

Pickel, J ;

McKay, R ;

Nadal-Ginard, B ;

Bodine, DM ;

Leri, A ;

Anversa, P .

NATURE, 2001, 410 (6829) :701-705

[28] Adult bone marrow stem cells regenerate myocardium in ischemic heart disease [J].

Orlic, D .

HEMATOPOIETIC STEM CELLS 2002: GENETICS AND FUNCTION, 2003, 996 :152-157

[29] Autologous hematopoietic stem cell transplantation for breast cancer in Europe: critical evaluation of data from the European Group for Blood and Marrow Transplantation (EBMT) Registry 1990-1999 [J].

Pedrazzoli, P ;

Ferrante, P ;

Kulekci, A ;

Schiavo, R ;

De Giorgi, U ;

Carminati, O ;

Marangolo, M ;

Demirer, T ;

Siena, S ;

Rosti, G .

BONE MARROW TRANSPLANTATION, 2003, 32 (05) :489-494

[30] Injection of adult neurospheres induces recovery in a chronic model of multiple sclerosis [J].

Pluchino, S ;

Quattrini, A ;

Brambilla, E ;

Gritti, A ;

Salani, G ;

Dina, G ;

Galli, R ;

Del Carro, U ;

Amadio, S ;

Bergami, A ;

Furlan, R ;

Comi, G ;

Vescovi, AL ;

Martino, G .

NATURE, 2003, 422 (6933) :688-694

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