Phorbol ester-sensitive phospholipase D is mainly localized in the endoplasmic reticulum of BHK cells

被引：16

作者：

Decker, C ^{[1
]}

Obradors, MJM ^{[1
]}

Sillence, DJ ^{[1
]}

Allan, D ^{[1
]}

机构：

[1] UNIV LONDON UNIV COLL,DEPT PHYSIOL,LONDON WC1E 6JJ,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1996年 / 320卷

关键词：

D O I：

10.1042/bj3200885

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The localization of phorbol ester-sensitive phospholipase D (PLD) in baby hamster kidney cells has been investigated by determining the subcellular distribution of the phosphatidylbutanol produced when the cells are incubated with phorbol 12-myristate 13-acetate and n-butanol. Results derived by isolation of plasma membrane vesicles from intact cells or by subcellular fractionation on a sucrose density gradient suggest the PLD is specific for phosphatidylcholine and its primary site of action is not the plasma membrane but the endoplasmic reticulum.

引用

页码：885 / 890

页数：6

共 29 条

[11] ACCUMULATION OF PHOSPHATIDYLALCOHOL IN CULTURED-CELLS - USE OF SUBCELLULAR FRACTIONATION TO INVESTIGATE PHOSPHOLIPASE-D ACTIVITY DURING SIGNAL-TRANSDUCTION [J].

EDWARDS, JS ;

MURRAY, AW .

BIOCHEMICAL JOURNAL, 1995, 308 :473-480

[12] 2 ENZYMES INVOLVED IN THE SYNTHESIS OF O-LINKED OLIGOSACCHARIDES ARE LOCALIZED ON MEMBRANES OF DIFFERENT DENSITIES IN MOUSE LYMPHOMA BW5147-CELLS [J].

ELHAMMER, A ;

KORNFELD, S .

JOURNAL OF CELL BIOLOGY, 1984, 99 (01) :327-331

[13] ASYMMETRY OF PHOSPHOLIPID BILAYER OF RAT-LIVER ENDOPLASMIC-RETICULUM [J].

HIGGINS, JA ;

DAWSON, RMC .

BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 470 (03) :342-356

[14] ANALYSIS OF THE PHOSPHOLIPID OF THE NUCLEAR-ENVELOPE AND ENDOPLASMIC-RETICULUM OF LIVER-CELLS BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY [J].

JAMES, JL ;

CLAWSON, GA ;

CHAN, CH ;

SMUCKLER, EA .

LIPIDS, 1981, 16 (07) :541-545

[15] ARF SIGNALING - A POTENTIAL ROLE FOR PHOSPHOLIPASE-D IN MEMBRANE TRAFFIC [J].

KAHN, RA ;

YUCEL, JK ;

MALHOTRA, V .

CELL, 1993, 75 (06) :1045-1048

[16] EFFECTS OF BREFELDIN-A ON SPHINGOMYELIN TRANSPORT AND LIPID-SYNTHESIS IN BHK-21-CELLS [J].

KALLEN, KJ ;

QUINN, P ;

ALLAN, D .

BIOCHEMICAL JOURNAL, 1993, 289 :307-312

[17] Phosphatidic acid mobilized by phospholipase D is involved in the phorbol 12-myristate 13-acetate-induced G(2) delay of A431 cells [J].

Kaszkin, M ;

Richards, J ;

Kinzel, V .

BIOCHEMICAL JOURNAL, 1996, 314 :129-138

[18]

KISS Z, 1989, J BIOL CHEM, V264, P1483

[19] PHOSPHOLIPASE-D IS PRESENT ON GOLGI-ENRICHED MEMBRANES AND ITS ACTIVATION BY ADP-RIBOSYLATION FACTOR IS SENSITIVE TO BREFELDIN-A [J].

KTISTAKIS, NT ;

BROWN, HA ;

STERNWEIS, PC ;

ROTH, MG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (11) :4952-4956

[20] TRANSVERSE ASYMMETRY OF PHOSPHOLIPIDS IN SUBCELLULAR MEMBRANES OF RAT-LIVER [J].

NILSSON, OS ;

DALLNER, G .

BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 464 (02) :453-458

← 1 2 3 →