Separation and quantitation of water soluble cellular metabolites by hydrophilic interaction chromatography-tandem mass spectrometry

被引：487

作者：

Bajad, Sunil U.

Lu, Wenyun

Kimball, Elizabeth H.

Yuan, Jie

Peterson, Celeste

Rabinowitz, Joshua D. ^{[1
]}

机构：

[1] Princeton Univ, Lewis Sigler Inst Integrat Genom, Princeton, NJ 08544 USA

[2] Princeton Univ, Dept Chem, Princeton, NJ 08544 USA

[3] Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1125卷 / 01期

基金：

美国国家卫生研究院;

关键词：

LC-ESI-MS/MS; Escherichia coli; metabolomics; metabonomics; hydrophilic interaction chromatography; bacteria; metabolite; metabolism; triple quadrupole; mass spectrometry;

D O I：

10.1016/j.chroma.2006.05.019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A key unmet need in metabolomics is the ability to efficiently quantify a large number of known cellular metabolites. Here we present a liquid chromatography (LC)-electrospray ionization tandem mass spectrometry (ESI-MS/MS) method for reliable measurement of 141 metabolites, including components of central carbon, amino acid, and nucleotide metabolism. The selected LC approach, hydrophilic interaction chromatography with an amino column, effectively separates highly water soluble metabolites that fail to retain using standard reversed-phase chromatography. MS/MS detection is achieved by scanning through numerous selected reaction monitoring events on a triple quadrupole instrument. When applied to extracts of Escherichia coli grown in [C-12]- versus [C-13]glucose, the method reveals appropriate C-12- and C-13-peaks for 79 different metabolites. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：76 / 88

页数：13

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