Membrane blebbing during apoptosis results from caspase-mediated activation of ROCK I

被引：1024

作者：

Coleman, ML

Sahai, EA

Yeo, M

Bosch, M

Dewar, A

Olson, MF

机构：

[1] Inst Canc Res, Chester Beatty Labs, CRC, Ctr Cellular & Mol Biol, London SW3 6JB, England

[2] Royal Brompton Hosp, Natl Heart & Lung Inst, London SW3 6NP, England

来源：

NATURE CELL BIOLOGY | 2001年 / 3卷 / 04期

关键词：

D O I：

10.1038/35070009

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The execution phase of apoptosis is characterized by marked changes in cell morphology that include contraction and membrane blebbing. The actin-myosin system has been proposed to be the source of contractile force that drives bleb formation, although the biochemical pathway that promotes actin-myosin contractility during apoptosis has not been identified. Here we show that the Rho effector protein ROCK I, which contributes to phosphorylation of myosin light-chains, myosin ATPase activity and coupling of actin-myosin filaments to the plasma membrane, is cleaved during apoptosis to generate a truncated active form. The activity of ROCK proteins is both necessary and sufficient for formation of membrane blebs and for re-localization of fragmented DNA into blebs and apoptotic bodies.

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收藏

页码：339 / 345

页数：7

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