Sorting of small RNAs into Arabidopsis argonaute complexes is directed by the 5′ terminal nucleotide

被引：1089

作者：

Mi, Shijun ^{[1
]}

Cai, Tao ^{[1
]}

Hu, Yugang ^{[1
]}

Chen, Yemiao ^{[1
]}

Hodges, Emily ^{[2
,3
]}

Ni, Fangrui ^{[1
]}

Wu, Liang ^{[1
]}

Li, Shan ^{[1
]}

Zhou, Huanyu ^{[1
]}

Long, Chengzu ^{[1
]}

Chen, She ^{[1
]}

Hannon, Gregory J. ^{[2
,3
]}

Qi, Yijun ^{[1
]}

机构：

[1] Natl Inst Biol Sci, Beijing 102206, Peoples R China

[2] Watson Sch Biol Sci, Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

[3] Howard Hughes Med Inst, Cold Spring Harbor, NY 11724 USA

来源：

CELL | 2008年 / 133卷 / 01期

关键词：

D O I：

10.1016/j.cell.2008.02.034

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Argonaute (AGO) proteins recruit small RNAs to form the core of RNAi effector complexes. Arabidopsis encodes ten AGO proteins and a large network of small RNAs. How these small RNAs are sorted into specific AGO complexes remains largely unknown. We have cataloged small RNAs resident in four AGO complexes. We found that AGO2 and AGO4 preferentially recruit small RNAs with a 5 ' terminal adenosine, whereas AGO1 harbors microRNAs (miRNAs) that favor a 5 ' terminal uridine. AGO5 predominantly binds small RNAs that initiate with cytosine. Changing the 5 ' terminal nucleotide of an miRNA predictably redirected it into a different AGO complex and alters its biological activity. These results reveal a role for small RNA sequences in assorting among AGO complexes. This suggests that specialization of AGO complexes might involve remodeling the 5 ' end-binding pocket to accept certain small RNA sequences, perhaps explaining the evolutionary drive for miRNAs to initiate with uridine.

引用

页码：116 / 127

页数：12

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