A physical map with yeast artificial chromosome (YAC) clones covering 63% of the 12 rice chromosomes

被引：37

作者：

Saji, S

Umehara, Y

Antonio, B

Yamane, H

Tanoue, H

Baba, T

Aoki, H

Ishige, N

Wu, JZ

Koike, K

Matsumoto, T

Sasaki, T

机构：

[1] Natl Inst Agrobiol Resources, Rice Genome Res Program, Tsukuba, Ibaraki 3058602, Japan

[2] Inst Soc Technoinnovat Agr Forestry & Fisheries, Rice Genome Res Program, Tsukuba, Ibaraki 3050854, Japan

来源：

GENOME | 2001年 / 44卷 / 01期

关键词：

physical mapping; YAC contig; rice genome; rice chromosomes;

D O I：

10.1139/gen-44-1-32

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new YAC (yeast artificial chromosome) physical map of the 12 rice chromosomes was constructed utilizing the latest molecular linkage map. The 1439 DNA markers on the rice genetic map selected a total of 1892 YACs from a YAC library. A total of 675 distinct YACs were assigned to specific chromosomal locations. In all chromosomes, 297 YAC contigs and 142 YAC islands were formed. The total physical length of these contigs and islands was estimated to 270 Mb which corresponds to approximately 63% of the entire rice genome (430 Mb). Because the physical length of each YAC contig has been measured, we could then estimate the physical distance between genetic markers more precisely than previously. In the course of constructing the new physical map, the DNA markers mapped at 0.0-cM intervals were ordered accurately and the presence of potentially duplicated regions among the chromosomes was detected. The physical map combined with the genetic map will form the basis for elucidation of the rice genome structure, map-based cloning of agronomically important genes, and genome sequencing.

引用

页码：32 / 37

页数：6

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