Cloning and expression of interleukin-18 binding protein

被引：112

作者：

Aizawa, Y ^{[1
]}

Akita, K ^{[1
]}

Taniai, M ^{[1
]}

Torigoe, K ^{[1
]}

Mori, T ^{[1
]}

Nishida, Y ^{[1
]}

Ushio, S ^{[1
]}

Nukada, Y ^{[1
]}

Tanimoto, T ^{[1
]}

Ikegami, H ^{[1
]}

Ikeda, M ^{[1
]}

Kurimoto, M ^{[1
]}

机构：

[1] Hayashibara Biochem Labs, Fujisaki Inst, Okayama 7028006, Japan

来源：

FEBS LETTERS | 1999年 / 445卷 / 2-3期

关键词：

interleukin-18 binding protein; interleukin-18; receptor; complex; antagonist;

D O I：

10.1016/S0014-5793(99)00148-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Interleukin-18 binding protein is a novel glycoprotein that we successfully cloned and expressed. First, murine interleukin-18 binding protein was purified from the sera of mice with endotoxin shock using ligand affinity chromatography. The murine interleukin-18 binding protein cDNA was cloned after RT-PCR using mixed primer pair sequences based on partial murine interleukin-18 binding protein amino acid sequence analysis. Subsequently, human interleukin-18 binding protein cDNA was cloned from cDNA libraries of normal human liver using murine interleukin-18 binding protein cDNA as a probe. Next, we transiently expressed recombinant human and murine interleukin-18 binding proteins in COS-1 cells and purified them from culture supernatants. Both recombinant interleukin-18 binding proteins did not exhibit species specificity and prevented interleukin-18 binding to its receptor. In addition, they inhibited interleukine-18 dependent IFN-gamma production from KG-1 cells effectively. These results suggest that the interleukin-18 binding protein may possess interleukine-18 antagonist activity. (C) 1999 Federation of European Biochemical Societies.

引用

页码：338 / 342

页数：5

共 18 条

[11] CLONING OF A NEW CYTOKINE THAT INDUCES IFN-GAMMA PRODUCTION BY T-CELLS [J].

OKAMURA, H ;

TSUTSUI, H ;

KOMATSU, T ;

YUTSUDO, M ;

HAKURA, A ;

TANIMOTO, T ;

TORIGOE, K ;

OKURA, T ;

NUKADA, Y ;

HATTORI, K ;

AKITA, K ;

NAMBA, M ;

TANABE, F ;

KONISHI, K ;

FUKUDA, S ;

KURIMOTO, M .

NATURE, 1995, 378 (6552) :88-91

[12] Systemic production of interferon-gamma inducing factor (IGIF) versus local IFN-gamma expression involved in the development of Th1 insulitis in NOD mice [J].

Rothe, H ;

Hibino, T ;

Itoh, Y ;

Kolb, H ;

Martin, S .

JOURNAL OF AUTOIMMUNITY, 1997, 10 (03) :251-256

[13]

Stoll S, 1997, J IMMUNOL, V159, P298

[14] Characterization of anti-human interleukin-18 (IL-18)/interferon-gamma-inducing factor (IGIF) monoclonal antibodies and their application in the measurement of human IL-18 by ELISA [J].

Taniguchi, M ;

Nagaoka, K ;

Kunikata, T ;

Kayano, T ;

Yamauchi, H ;

Nakamura, S ;

Ikeda, M ;

Orita, K ;

Kurimoto, M .

JOURNAL OF IMMUNOLOGICAL METHODS, 1997, 206 (1-2) :107-113

[15] Purification and characterization of the human interleukin-18 receptor [J].

Torigoe, K ;

Ushio, S ;

Okura, T ;

Kobayashi, S ;

Taniai, M ;

Kunikata, T ;

Murakami, T ;

Sanou, O ;

Kojima, H ;

Fujii, M ;

Ohta, T ;

Ikeda, M ;

Ikegami, H ;

Kurimoto, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (41) :25737-25742

[16]

Tsutsui H, 1997, J IMMUNOL, V159, P3961

[17]

Tsutsui H, 1996, J IMMUNOL, V157, P3967

[18]

Ushio S, 1996, J IMMUNOL, V156, P4274

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