Cloning and expression of interleukin-18 binding protein

被引：112

作者：

Aizawa, Y ^{[1
]}

Akita, K ^{[1
]}

Taniai, M ^{[1
]}

Torigoe, K ^{[1
]}

Mori, T ^{[1
]}

Nishida, Y ^{[1
]}

Ushio, S ^{[1
]}

Nukada, Y ^{[1
]}

Tanimoto, T ^{[1
]}

Ikegami, H ^{[1
]}

Ikeda, M ^{[1
]}

Kurimoto, M ^{[1
]}

机构：

[1] Hayashibara Biochem Labs, Fujisaki Inst, Okayama 7028006, Japan

来源：

FEBS LETTERS | 1999年 / 445卷 / 2-3期

关键词：

interleukin-18 binding protein; interleukin-18; receptor; complex; antagonist;

D O I：

10.1016/S0014-5793(99)00148-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Interleukin-18 binding protein is a novel glycoprotein that we successfully cloned and expressed. First, murine interleukin-18 binding protein was purified from the sera of mice with endotoxin shock using ligand affinity chromatography. The murine interleukin-18 binding protein cDNA was cloned after RT-PCR using mixed primer pair sequences based on partial murine interleukin-18 binding protein amino acid sequence analysis. Subsequently, human interleukin-18 binding protein cDNA was cloned from cDNA libraries of normal human liver using murine interleukin-18 binding protein cDNA as a probe. Next, we transiently expressed recombinant human and murine interleukin-18 binding proteins in COS-1 cells and purified them from culture supernatants. Both recombinant interleukin-18 binding proteins did not exhibit species specificity and prevented interleukin-18 binding to its receptor. In addition, they inhibited interleukine-18 dependent IFN-gamma production from KG-1 cells effectively. These results suggest that the interleukin-18 binding protein may possess interleukine-18 antagonist activity. (C) 1999 Federation of European Biochemical Societies.

引用

页码：338 / 342

页数：5

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