The golgi-associated protein GRASP is required for unconventional protein secretion during development

被引：199

作者：

Kinseth, Matthew A. ^{[1
]}

Anjard, Christophe ^{[1
]}

Fuller, Danny ^{[1
]}

Guizzunti, Gianni ^{[1
]}

Loomis, William F. ^{[1
]}

Malhotra, Vivek ^{[1
]}

机构：

[1] Univ Calif San Diego, Div Biol Sci, La Jolla, CA 92093 USA

来源：

CELL | 2007年 / 130卷 / 03期

关键词：

D O I：

10.1016/j.cell.2007.06.029

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

During Dictyostelium development, prespore cells secrete acyl-CoA binding protein (AcbA). Upon release, AcbA is processed to generate a peptide called spore differentiation factor-2 (SDF-2), which triggers terminal differentiation of spore cells. We have found that cells lacking Golgi reassembly stacking protein (GRASP), a protein attached peripherally to the cytoplasmic surface of Golgi membranes, fail to secrete AcbA and, thus, produce inviable spores. Surprisingly, AcbA lacks a signal sequence and is not secreted via the conventional secretory pathway (endoplasmic reticulum-Golgi-cell surface). GRASP is not required for conventional protein secretion, growth, and the viability of vegetative cells. Our findings reveal a physiological role of GRASP and provide a means to understand unconventional secretion and its role in development.

引用

页码：524 / 534

页数：11

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