Expression profiling using cDNA microarrays

被引：1192

作者：

Duggan, DJ ^{[1
]}

Bittner, M ^{[1
]}

Chen, YD ^{[1
]}

Meltzer, P ^{[1
]}

Trent, JM ^{[1
]}

机构：

[1] NHGRI, Canc Genet Branch, NIH, Bethesda, MD 20892 USA

来源：

NATURE GENETICS | 1999年 / 21卷 / Suppl 1期

关键词：

D O I：

10.1038/4434

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

cDNA microarrays are capable of profiling gene expression patterns of tens of thousands of genes in a single experiment. DNA targets, in the form of 3' expressed sequence tags (ESTs), are arrayed onto glass slides (or membranes) and probed with fluorescent- or radioactively-labelled cDNAs. Here,we review technical aspects of cDNA microarrays, including the general principles, fabrication of the arrays, target labelling, image analysis and data extraction, management and mining.

引用

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页码：10 / 14

页数：5

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