Carbon nanotubes as intracellular protein transporters: Generality and biological functionality

被引:827
作者
Kam, NWS
Dai, HJ [1 ]
机构
[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA
[2] Stanford Univ, Adv Mat Lab, Stanford, CA 94305 USA
关键词
D O I
10.1021/ja050062v
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Various proteins adsorb spontaneously on the sidewalls of acid-oxidized single-walled carbon nanotubes. This simple nonspecific binding scheme can be used to afford noncovalent protein-nanotube conjugates. The proteins are found to be readily transported inside various mammalian cells with nanotubes acting as the transporter via the endocytosis pathway. Once released from the endosomes, the internalized protein-nanotube conjugates can enter into the cytoplasm of cells and perform biological functions, evidenced by apoptosis induction by transported cytochrome c. Carbon nanotubes represent a new class of molecular transporters potentially useful for future in vitro and in vivo protein delivery applications.
引用
收藏
页码:6021 / 6026
页数:6
相关论文
共 26 条
[21]   Clathrin-coated vesicle formation and protein sorting: An integrated process [J].
Schmid, SL .
ANNUAL REVIEW OF BIOCHEMISTRY, 1997, 66 :511-548
[22]   Functionalization of carbon nanotubes for biocompatibility and biomolecular recognition [J].
Shim, M ;
Kam, NWS ;
Chen, RJ ;
Li, YM ;
Dai, HJ .
NANO LETTERS, 2002, 2 (04) :285-288
[23]   ENDOCYTOSIS [J].
SILVERSTEIN, SC ;
STEINMAN, RM ;
COHN, ZA .
ANNUAL REVIEW OF BIOCHEMISTRY, 1977, 46 :669-722
[24]   Pharmacokinetics and metabolism in early drug discovery [J].
Smith, DA ;
van de Waterbeemd, H .
CURRENT OPINION IN CHEMICAL BIOLOGY, 1999, 3 (04) :373-378
[25]   A NEW VITAL STAIN FOR VISUALIZING VACUOLAR MEMBRANE DYNAMICS AND ENDOCYTOSIS IN YEAST [J].
VIDA, TA ;
EMR, SD .
JOURNAL OF CELL BIOLOGY, 1995, 128 (05) :779-792
[26]   Injected cytochrome c induces apoptosis [J].
Zhivotovsky, B ;
Orrenius, S ;
Brustugun, OT ;
Doskeland, SO .
NATURE, 1998, 391 (6666) :449-450