The p400 complex is an essential E1A transformation target

被引：255

作者：

Fuchs, M

Gerber, J

Drapkin, R

Sif, S

Ikura, T

Ogryzko, V

Lane, WS

Nakatani, Y

Livingston, DM ^{[1
]}

机构：

[1] Dana Farber Canc Inst, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Boston, MA USA

[3] Brigham & Womens Hosp, Dept Pathol, Boston, MA 02115 USA

[4] Ohio State Univ, Sch Med, Columbus, OH 43210 USA

[5] Harvard Univ, Microchem & Proteom Anal Facil, Cambridge, MA 02138 USA

[6] Inst Andre Lwoff, Villejuif, France

来源：

CELL | 2001年 / 106卷 / 03期

关键词：

D O I：

10.1016/S0092-8674(01)00450-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here, we report the identification of a new E1A binding protein complex that is essential for E1A-mediated transformation. Its core component is a SWI2/SNF2-related, 400 kDa protein (p400). Other components include the myc- and p/CAF-associated cofactor, TRRAP/PAF400, the DNA helicases TAP54 alpha/beta, actin-like proteins, and the human homolog of the Drosophila Enhancer of Polycomb protein. An E1A mutant, defective in p400 binding, is also defective in transformation. Certain p400 fragments partially rescued this phenotype, underscoring the role of E1A-p400 complex formation in the E1A transforming process. Furthermore, E1A and c-myc each alter the subunit composition of p400 complexes, implying that physiological p400 complex formation contributes to transformation suppression.

引用

页码：297 / 307

页数：11

共 36 条

[1] NuA4, an essential transcription adaptor/histone H4 acetyltransferase complex containing Esa1p and the ATM-related cofactor Tra1p [J].