Two actin-related proteins are shared functional components of the chromatin-remodeling complexes RSC and SWI/SNF

被引:177
作者
Cairns, BR [1 ]
Erdjument-Bromage, H
Tempst, P
Winston, F
Kornberg, RD
机构
[1] Stanford Univ, Sch Med, Dept Biol Struct, Stanford, CA 94305 USA
[2] Mem Sloan Kettering Canc Ctr, Program Mol Biol, New York, NY 10021 USA
[3] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA
[4] Univ Utah, Sch Med, Huntsman Canc Inst, Salt Lake City, UT 84108 USA
[5] Univ Utah, Sch Med, Dept Oncol Sci, Salt Lake City, UT 84108 USA
关键词
D O I
10.1016/S1097-2765(00)80162-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The yeast Saccharomyces cerevisiae contains two related chromatin-remodeling complexes, RSC and SWI/SNF, which are shown to share the actin-related proteins Arp7 and Arp9. Depending on the genetic background tested, arp7 Delta and arp9 Delta mutants are either inviable or show greatly impaired growth and Swi(-)/Snf(-) mutant phenotypes. Unlike swi(-)/snf(-) mutants, viable arp7 Delta or arp9 Delta mutants have an Spt(-) phenotype, suggesting that RSC affects transcription. Temperature-sensitive mutations in ARP7 and ARP9 were isolated, and the amino acid changes support the structural relationship of Arp7 and Arp9 to actin. However, site-directed mutations predicted to impair ATP binding or hydrolysis did not detectably affect Arp7 or Arp9 function. Our results suggest that actin-related proteins perform important roles in chromatin-remodeling complexes by virtue of structural rather than enzymatic similarities to actin.
引用
收藏
页码:639 / 651
页数:13
相关论文
共 52 条
[1]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[2]  
Cairns BR, 1996, MOL CELL BIOL, V16, P3308
[3]   Essential role of Swp73p in the function of yeast Swi/Snf complex [J].
Cairns, BR ;
Levinson, RS ;
Yamamoto, KR ;
Kornberg, RD .
GENES & DEVELOPMENT, 1996, 10 (17) :2131-2144
[4]   RSC, an essential, abundant chromatin-remodeling complex [J].
Cairns, BR ;
Lorch, Y ;
Li, Y ;
Zhang, MC ;
Lacomis, L ;
ErdjumentBromage, H ;
Tempst, P ;
Du, J ;
Laurent, B ;
Kornberg, RD .
CELL, 1996, 87 (07) :1249-1260
[5]   A MULTISUBUNIT COMPLEX CONTAINING THE SWI1/ADR6, SWI2/SNF2, SWI3, SNF5, AND SNF6 GENE-PRODUCTS ISOLATED FROM YEAST [J].
CAIRNS, BR ;
KIM, YJ ;
SAYRE, MH ;
LAURENT, BC ;
KORNBERG, RD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (05) :1950-1954
[6]   Sfh1p, a component of a novel chromatin-remodeling complex, is required for cell cycle progression [J].
Cao, YX ;
Cairns, BR ;
Kornberg, RD ;
Laurent, BC .
MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (06) :3323-3334
[7]   A MUTATION IN AN ATP-BINDING LOOP OF SACCHAROMYCES-CEREVISIAE ACTIN (S14A) CAUSES A TEMPERATURE-SENSITIVE PHENOTYPE IN-VIVO AND IN-VITRO [J].
CHEN, X ;
RUBENSTEIN, PA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (19) :11406-11414
[8]   STIMULATION OF GAL4 DERIVATIVE BINDING TO NUCLEOSOMAL DNA BY THE YEAST SWI/SNF COMPLEX [J].
COTE, J ;
QUINN, J ;
WORKMAN, JL ;
PETERSON, CL .
SCIENCE, 1994, 265 (5168) :53-60
[9]   SPT15, THE GENE ENCODING THE YEAST TATA BINDING-FACTOR TFIID, IS REQUIRED FOR NORMAL TRANSCRIPTION INITIATION INVIVO [J].
EISENMANN, DM ;
DOLLARD, C ;
WINSTON, F .
CELL, 1989, 58 (06) :1183-1191
[10]   IDENTIFICATION AND CHARACTERIZATION OF DROSOPHILA RELATIVES OF THE YEAST TRANSCRIPTIONAL ACTIVATOR SNF2/SWI2 [J].
ELFRING, LK ;
DEURING, R ;
MCCALLUM, CM ;
PETERSON, CL ;
TAMKUN, JW .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (04) :2225-2234