Selective binding of TAR RNA by a tat-derived β-peptide

被引：65

作者：

Gelman, MA ^{[1
]}

Richter, S

Cao, H

Umezawa, N

Gellman, SH

Rana, TM

机构：

[1] Univ Wisconsin, Dept Chem, Madison, WI 53706 USA

[2] Univ Wisconsin, Med Sci Training Program, Madison, WI 53706 USA

[3] Univ Massachusetts, Sch Med, Dept Mol Pharmacol & Biochem, Chem Biol Program, Worcester, MA 01605 USA

来源：

ORGANIC LETTERS | 2003年 / 5卷 / 20期

关键词：

D O I：

10.1021/ol034977v

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The interaction between the HIV-1 Tat protein and the TAR RNA element in the nascent viral genomic transcript is required for viral replication. An 11-residue beta-peptide (1), an all-beta homologue of the Arg-rich region Tat 47-57, binds TAR RNA with K-d = 29 +/- 4 nM. A control beta-peptide (2) in which all Arg side chains are replaced by Lys side chains shows increased affinity but decreased specificity for wild-type vs bulge-deleted TAR RNA, as do the alpha-peptide analogues of 1 and 2.

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页码：3563 / 3565

页数：3

相关论文

共 27 条

[1] ARGININE-MEDIATED RNA RECOGNITION - THE ARGININE FORK [J].

CALNAN, BJ ;

TIDOR, B ;

BIANCALANA, S ;

HUDSON, D ;

FRANKEL, AD .

SCIENCE, 1991, 252 (5009) :1167-1171

[2] ANALYSIS OF ARGININE-RICH PEPTIDES FROM THE HIV TAT PROTEIN REVEALS UNUSUAL FEATURES OF RNA PROTEIN RECOGNITION [J].

CALNAN, BJ ;

BIANCALANA, S ;

HUDSON, D ;

FRANKEL, AD .

GENES & DEVELOPMENT, 1991, 5 (02) :201-210

[3] β-peptides:: From structure to function [J].

Cheng, RP ;

Gellman, SH ;

DeGrado, WF .

CHEMICAL REVIEWS, 2001, 101 (10) :3219-3232

[4] HIGH-AFFINITY BINDING OF TAR RNA BY THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 TAT PROTEIN REQUIRES BASE-PAIRS IN THE RNA STEM AND AMINO-ACID-RESIDUES FLANKING THE BASIC REGION [J].

CHURCHER, MJ ;

LAMONT, C ;

HAMY, F ;

DINGWALL, C ;

GREEN, SM ;

LOWE, AD ;

BUTLER, PJG ;

GAIT, MJ ;

KARN, J .

JOURNAL OF MOLECULAR BIOLOGY, 1993, 230 (01) :90-110

[5] SEQUENCE-SPECIFIC INTERACTION OF TAT PROTEIN AND TAT PEPTIDES WITH THE TRANSACTIVATION-RESPONSIVE SEQUENCE ELEMENT OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 INVITRO [J].

CORDINGLEY, MG ;

LAFEMINA, RL ;

CALLAHAN, PL ;

CONDRA, JH ;

SARDANA, VV ;

GRAHAM, DJ ;

NGUYEN, TM ;

LEGROW, K ;

GOTLIB, L ;

SCHLABACH, AJ ;

COLONNO, RJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (22) :8985-8989

[6] HIV-1 TAT PROTEIN STIMULATES TRANSCRIPTION BY BINDING TO A U-RICH BULGE IN THE STEM OF THE TAR RNA STRUCTURE [J].

DINGWALL, C ;

ERNBERG, I ;

GAIT, MJ ;

GREEN, SM ;

HEAPHY, S ;

KARN, J ;

LOWE, AD ;

SINGH, M ;

SKINNER, MA .

EMBO JOURNAL, 1990, 9 (12) :4145-4153

[7]

Gademann K, 1999, ANGEW CHEM INT EDIT, V38, P1223, DOI 10.1002/(SICI)1521-3773(19990503)38:9<1223::AID-ANIE1223>3.0.CO

[8]

2-A

[9]

Gademann K, 1999, CURR MED CHEM, V6, P905

[10] Homonuclear 1H-NMR Assignment and Structural Characterization of Human Immunodeficiency Virus Type 1 Tat Mal Protein [J].

Grégoire, C ;

Peloponese, JM ;

Esquieu, D ;

Opi, S ;

Campbell, G ;

Solomiac, M ;

Lebrun, E ;

Lebreton, J ;

Loret, EP .

BIOPOLYMERS, 2001, 62 (06) :324-335