Molecular properties of apelin: tissue distribution and receptor binding

被引：458

作者：

Kawamata, Y ^{[1
]}

Fukusumi, S ^{[1
]}

Hosoya, M ^{[1
]}

Fujii, R ^{[1
]}

Hinuma, S ^{[1
]}

Nishizawa, N ^{[1
]}

Kitada, C ^{[1
]}

Onda, H ^{[1
]}

Nishimura, O ^{[1
]}

Fujino, M ^{[1
]}

机构：

[1] Takeda Chem Ind Ltd, Pharmaceut Discovery Res Div, Discovery Res Labs 1, Tsukuba, Ibaraki 3004293, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 2001年 / 1538卷 / 2-3期

关键词：

apelin; APJ; distribution; antibody; receptor binding;

D O I：

10.1016/S0167-4889(00)00143-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We analyzed the tissue distribution of apelin mRNA in rats by a quantitative revere transcription-polymerase chain reaction and that of immunoreactive apelin (ir-apelin) by an enzyme immunoassay (EIA) using a monoclonal antibody. The expression levels of apelin mRNA and ir-apelin seemed to be consistent among tissues: they were highly expressed in the lung and mammary gland. By the combination of gel filtration and EIA, nle found that the molecular forms of apelin differ among respective tissues: apelin molecules with sizes close to apelin-36 (long forms) were major components ill the lung, testis, and uterus, but both long and short (whose sizes were close to [< Glu(65)]apelin-13) forms were detected in the mammary gland. In Scatchard analyses, the radioiodinated apelin-36 analogue bound to the receptor. APJ, with high affinity. In competitive binding assays, apelin-36 and apelin-19 far more efficiently inhibited the binding of the labeled apelin-36 analogue with APJ than [< Glu(65)]apelin-13. In analyses for the dissociation of apelin from APJ, unlabeled apelin-36 replaced mole rapidly the labeled apelin-36 analogue bound with APJ than [ < Glu(65)]apelin-13. Our results demonstrate that the long and short forms of apelin differently interact with APJ. <(c)> Elsevier Science B.V. All rights reserved.

引用

页码：162 / 171

页数：10

共 19 条

[11] Characterization of apelin, the ligand for the APJ receptor [J].