Environmental regulation of Salmonella pathogenicity island 2 gene expression

被引：303

作者：

Deiwick, J ^{[1
]}

Nikolaus, T ^{[1
]}

Erdogan, S ^{[1
]}

Hensel, M ^{[1
]}

机构：

[1] Max Von Pettenkofer Inst Hyg & Med Microbiol, Lehrstuhl Bakteriol, D-80336 Munich, Germany

来源：

MOLECULAR MICROBIOLOGY | 1999年 / 31卷 / 06期

关键词：

D O I：

10.1046/j.1365-2958.1999.01312.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The enteric pathogen Salmonella typhimurium co-ordinates the expression of virulence determinants in response to environmental cues from the host organism. S. typhimurium possesses Salmonella pathogenicity island 2 (SP12), a large virulence locus encoding a type III secretion system for virulence determinants required for systemic infections and accumulation inside host cells. We have generated transcriptional fusions to SP12 genes to analyse expression and used antibodies against recombinant SP12 proteins to monitor levels of SP12 proteins under various conditions. Here, we demonstrate that SP12 gene expression is induced by Mg2+ deprivation and phosphate starvation. These conditions are likely to represent the environmental cues encountered by S. typhimurium inside the phagosome of infected host cells. The induction of SP12 gene expression is modulated by the global regulatory system PhoPQ and is dependent on SsrAB, a two-component regulatory system encoded by SP12.

引用

页码：1759 / 1773

页数：15

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