Effect of caffeic acid phenethyl ester, an antioxidant from propolis, on inducing apoptosis in human leukemic HL-60 cells

被引：127

作者：

Chen, YJ

Shiao, MS

Hsu, ML

Tsai, HT

Wang, SY

机构：

[1] Vet Gen Hosp, Dept Med Res & Educ, Taipei 11217, Taiwan

[2] Natl Yang Ming Univ, Inst Tradit Med, Taipei 11221, Taiwan

[3] Mackay Mem Hosp, Dept Radiat Oncol, Taipei 10449, Taiwan

[4] Chinese Culture Univ, Dept Martial Arts, Taipei 11114, Taiwan

[5] Natl Res Inst Chinese Med, Dept Pharmacol, Taipei 11211, Taiwan

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2001年 / 49卷 / 11期

关键词：

caffeic acid phenethyl ester (CAPE); apoptosis; caspase-3; Bcl-2; Bax;

D O I：

10.1021/jf0107252

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Caffeic acid phenethyl ester (CAPE) is an active component isolated from propolis, The aim of this study was to investigate the mechanism of CAPE-induced apoptosis in human leukemic HL-60 cells. It was found that CAPE entered HL-60 cells very quickly and then inhibited their survival in a concentration- and time-dependent manner. CAPE induced characteristic DNA fragmentation and morphological changes typical of apoptosis in these cells. Estimation of the apoptotic percentage showed a time-dependent increase after CAPE (6 mug/mL) treatment (up to 66.7 +/- 2.0% at 72 h). Treatment with CAPE caused rapid activation of caspase-3 after 4 h, down-regulation of Bcl-2 expression after 6 h, and up-regulation of Bax expression after 16 h. These results suggest that CAPE is a potent apoptosis-inducing agent; its action is accompanied by activation of caspase-3, down-regulation of Bcl-2, and up-regulation of Bax in human leukemic HL-60 cells.

引用

页码：5615 / 5619

页数：5